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Microbiology Ch.5

Total questions: 45

Worksheet time: 23mins

Name
Class
Date
1.

Which statements are true?

a)

ATP is used for the long-term storage of energy and so is often found in storage granules.

b)

ATP is a common intermediate between catabolic and anabolic pathways.

c)

2, 4, 5

d)

Electron carriers are located at ribosomes.

e)

Anaerobic organisms are capable of respiration.

2.

What is the fate of pyruvic acid in an organism that uses aerobic respiration?

a)

It is oxidized in the Krebs cycle.

b)

It is reduced in the Krebs cycle.

c)

It is oxidized in the electron transport chain.

d)

It is catabolized in glycolysis.

e)

It is reduced to lactic acid.

3.

A bacterial culture grown in a glucose-peptide medium causes the pH to increase. The bacteria are most likely

a)

Using the peptides.

b)

Oxidizing the glucose.

c)

Not growing.

d)

Fermenting the glucose.

4.

Which of the following statements about photophosphorylation is false?

a)

The oxidation of carrier molecules releases energy.

b)

It occurs in photosynthesizing cells.

c)

It requires CO2.

d)

Energy from oxidation reactions is used to generate ATP from ADP.

e)

Light liberates an electron from chlorophyll.

5.

In green and purple bacteria, electrons to reduce CO2 come from

a)

Chlorophyll.

b)

Sunlight.

c)

C6H12O6.

d)

H2.

e)

CO2.

6.

Which of the following is the best definition of oxidative phosphorylation?

a)

The energy released in the reduction of carrier molecules is used to generate ATP.

b)

The transfer of a high-energy phosphate group to ADP.

c)

The energy released as carrier molecules are oxidized is used to generate ATP.

d)

Electrons are passed through a series of carriers to O2.

7.

Which of the following statements about anaerobic respiration is false?

a)

It generates ATP.

b)

It involves the reduction of nitrate.

c)

It involves the Krebs cycle.

d)

It involves glycolysis only.

e)

It requires cytochromes.

8.

Gallionella bacteria can get energy from the reaction Fe2+ --> Fe3+. This reaction is an example of

a)

Oxidation.

b)

Fermentation.

c)

Photophosphorylation.

d)

The Calvin-Benson cycle.

e)

Reduction.

9.

Which of the following statements about substrate-level phosphorylation is false?

a)

The oxidation of intermediate metabolic compounds releases energy that is used to generate ATP.

b)

It involves the direct transfer of a high-energy phosphate group from an intermediate metabolic compound to ADP.

c)

No final electron acceptor is required.

d)

It occurs in glycolysis.

e)

It occurs in the Krebs cycle.

10.

In noncyclic photophosphorylation, O2 is produced from

a)

H2O.

b)

Sunlight.

c)

CO2.

d)

C6H12O6.

e)

Chlorophyll.

11.

Which of the following has bacteriochlorophylls and uses alcohols for carbon?

a)

Photoheterotroph

b)

Photoautotroph

c)

Chemoautotroph

d)

Chemoheterotroph

12.

Beggiatoa bacteria get energy by oxidizing S2- to S6+. This means they take ________ for their ________.

a)

electrons; electron transport chain

b)

glucose; glycolysis

c)

protons; NAD+

d)

electrons; fermentation

e)

sulfur; photophosphorylation

13.

Fatty acids are catabolized in

a)

Glycolysis.

b)

The pentose phosphate pathway.

c)

The Krebs cycle.

d)

The Entner Doudoroff pathway.

e)

The electron transport chain.

14.

The advantage of the pentose phosphate pathway is that it produces all of the following EXCEPT

a)

Precursors for the synthesis of amino acids.

b)

NADPH.

c)

Precursors for nucleic acids.

d)

Precursors for the synthesis of glucose.

e)

Three ATPs.

15.

Which of the following statements about beta oxidation is false?

a)

It is used in petroleum degradation.

b)

It involves the formation of acetyl-CoA.

c)

It involves the formation of 2-carbon units.

d)

It is a step in glycolysis.

e)

It is a method of catabolizing fatty acids.

16.

Which one of the following would you predict is an allosteric inhibitor of the Krebs cycle enzyme, α-ketoglutarate dehydrogenase?

a)

ADP

b)

-ketoglutaric acid

c)

Citric acid

d)

NAD+

e)

NADH

17.

Which of the following is the best definition of fermentation?

a)

The complete catabolism of glucose to CO2 and H2O

b)

The oxidation of glucose with organic molecules serving as electron acceptors

c)

The reduction of glucose to pyruvic acid

d)

The production of ethanol from glucose

e)

The production of energy by substrate-level phosphorylation

18.

Which of the following is NOT necessary for respiration?

a)

Quinones

b)

Flavoproteins

c)

Cytochromes

d)

Oxygen

e)

A source of electrons

19.

Which of the following uses CO2 for carbon and H2 for energy?

a)

Photoautotroph

b)

Photoheterotroph

c)

Chemoautotroph

d)

Chemoheterotroph

20.

Which of the following compounds is NOT an enzyme?

a)

Sucrase

b)

β-galactosidase

c)

Coenzyme A

d)

Dehydrogenase

e)

Cellulase

21.

A shipping company employee notices that the inside of ships' hulls where ballast water is stored are deteriorating. The hull paint contained cyanide to prevent microbial growth. Bacteria were growing on the hulls. You can therefore conclude that the

a)

Bacteria were using cytochromes.

b)

Bacteria were using aerobic respiration.

c)

Bacteria were growing by fermentation.

d)

Bacteria were using anaerobic respiration.

e)

Bacteria were photosynthetic.

22.

Assume you are growing bacteria on a lipid medium that started at pH 7. The action of bacterial lipases should cause the pH of the medium to

a)

Stay the same.

b)

Decrease.

c)

Increase.

23.

Cyanobacteria are a type of

a)

Photoheterotroph.

b)

Photoautotroph.

c)

Chemoheterotroph.

d)

Chemoautotroph.

24.

Which of the following uses glucose for carbon and energy?

a)

Photoautotroph

b)

Chemoautotroph

c)

Chemoheterotroph

d)

Photoheterotroph

25.

Which organism is NOT correctly matched to its energy source?

a)

Chemoautotroph - NH3

b)

Photoheterotroph - light

c)

Photoautotroph - CO2

d)

Chemoheterotroph - glucose

e)

Chemoautotroph - Fe2+

26.

A strictly fermentative bacterium produces energy

a)

Only in the absence of oxygen.

b)

By fermentation or aerobic respiration.

c)

Only in the presence of oxygen.

d)

By aerobic respiration only.

e)

By glycolysis only.

27.

Assume you are working for a chemical company and are responsible for growing a yeast culture that produces ethanol. The yeasts are growing well on the maltose medium but are not producing alcohol. What is the most likely explanation?

a)

The temperature is too low.

b)

The maltose is toxic.

c)

The temperature is too high.

d)

Not enough protein is provided.

e)

O2 is in the medium.

28.

An enzyme, citrate synthase, in the Krebs cycle is inhibited by ATP. This is an example of all of the following EXCEPT

a)

Noncompetitive inhibition.

b)

Beta oxidation.

c)

Feedback inhibition.

d)

Competitive inhibition.

e)

Allosteric inhibition.

29.

What is the most acidic place in Figure 5.8?

a)

a

b)

b

c)

c

d)

d

e)

e

30.
a)

This process occurs anaerobically.

b)

This process requires O2.

31.

In Figure 5.8, the structure labeled (1) is

a)

 

Cytoplasm.

b)

 

ATP synthase.

c)

 

Cell wall.

d)

 

NAD+.

e)

 

Plasma membrane.

32.

In Figure 5.8, where is ATP produced?

a)

a

b)

b

c)

c

d)

d

e)

e

33.

What type of reaction is in Figure 5.2?

a)

Oxidation

b)

Dehydrogenation

c)

Decarboxylation

d)

Reduction

e)

Transamination

34.

Which of the graphs in Figure 5.5 best illustrates the activity of an enzyme that is saturated with substrate?

a)

a

b)

b

c)

c

d)

d

e)

e

35.

How would a noncompetitive inhibitor interfere with a reaction involving the enzyme shown in Figure 5.3?

a)

 

It would bind to d.

b)

 

It would bind to a.

c)

 

Can't tell.

d)

 

It would bind to c.

e)

 

It would bind to b.

36.

Which compound is being reduced in the reaction shown in Figure 5.1?

a)

 


α
-ketoglutaric acid and NAD+

b)


Isocitric acid and α-ketoglutaric acid

c)

 

NADH and isocitric acid

d)

 

NADH

e)

 

NAD+

37.

A urease test is used to identify Myobacterium tuberculosis because

a)

 

Urea accumulates during tuberculosis.

b)

 

M. tuberculosis produces urease.

c)

 

Some bacteria reduce nitrate ion.

d)

 

Urease is a sign of tuberculosis.

e)

 

M. bovis can cause tuberculosis.

38.

Refer to Figure 5.8. In aerobic respiration, where is water formed?

a)

a

b)

b

c)

c

d)

d

e)

e

39.

In Figure 5.8, the path labeled (2) is the flow of

a)

 

Water.

b)

 

Glucose.

c)

 

Protons.

d)

 

Electrons.

e)

 

Energy.

40.
a)

This process requires O2.

b)

This process occurs anaerobically.

41.

The rates of O2 and glucose consumption by a bacterial culture are shown in Figure 5.6. Assume a bacterial culture was grown in a glucose medium without O2. Then O2 was added at the time marked X. The data indicate that

a)

 

These bacteria don't use O2.

b)

 

These bacteria can't grow anaerobically.

c)

 

Aerobic metabolism is more efficient than fermentation.

d)

 

These bacteria get more energy anaerobically.

42.

How is ATP generated in the reaction shown in Figure 5.4?

a)

 

Oxidative phosphorylation

b)

 

Glycolysis

c)

 

Photophosphorylation

d)

 

Substrate-level phosphorylation

e)

 

Fermentation

43.

The graph at the left in Figure 5.7 shows the reaction rate for an enzyme at its optimum temperature. Which graph shows enzyme activity at a higher temperature?

a)

a

b)

b

c)

c

d)

d

e)

e

44.
a)

This process requires O2.

b)

 

This process occurs anaerobically.

45.
a)

 

This process requires O2.

b)

 

This process occurs anaerobically.