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W1 Scientific Equipment and Techniques Quiz

Total questions: 106

Worksheet time: 2hrs 28mins

Name
Class
Date
1.

การใช้งานหลักของตู้บ่มคืออะไร?

a)

การเพาะเลี้ยงสิ่งมีชีวิตขนาดเล็ก เช่น แบคทีเรีย เชื้อรา หรือเซลล์สัตว์

b)

การควบคุมอุณหภูมิในช่วงการเจริญเติบโตที่เหมาะสม

c)

การรักษาความชื้นและระดับ CO₂ สำหรับการเพาะเลี้ยงเซลล์

2.

What is an example of using an incubator in a laboratory?

a)

Cultivating bacteria in culture media

b)

Maintaining a constant temperature for samples

3.

What is the purpose of using an incubator in bacterial culture?

a)

To maintain a constant temperature

b)

To mix solutions

c)

To create a flow of nutrients and air

4.

What is the function of a shaker?

a)

To maintain a constant temperature

b)

To mix solutions

c)

To create a flow of nutrients and air

5.

What are the advantages of using an incubator shaker?

a)

Increases oxygen solubility in culture media

b)

Helps microorganisms grow faster

c)

Reduces sediment formation in solutions

6.

การพาสเจอไรซ์คืออะไร?

a)

กระบวนการที่ใช้ความร้อนในการฆ่าเชื้อโรค

b)

วิธีการแช่แข็งอาหาร

c)

เทคนิคในการทำให้ผลไม้แห้ง

7.

การพาสเจอไรซ์คืออะไร?

a)

กระบวนการที่ใช้ความร้อนเพื่อฆ่าเชื้อโรคที่เป็นอันตราย

b)

วิธีการแช่แข็งอาหาร

c)

เทคนิคการทำให้ผลไม้แห้ง

d)

วิธีการบรรจุอาหาร

8.

What is the purpose of an autoclave?

a)

To sterilize equipment using high-pressure steam

b)

To cook food

c)

To store chemicals

d)

To cool down liquids

9.

What is the temperature used in an autoclave?

a)

Above 100°C

b)

Below 50°C

c)

Exactly 100°C

d)

Around 200°C

10.

What is the function of a hot-air oven?

a)

To sterilize equipment using dry heat

b)

To boil liquids

c)

To freeze food

d)

To mix ingredients

11.

What is a hot-air oven used for?

a)

To sterilize heat-resistant equipment

b)

To bake chemicals

c)

To remove moisture from substances

12.

What principle does a centrifuge operate on?

a)

Centrifugal force

b)

Gravitational force

c)

Magnetic force

13.

What happens to components with higher density in a centrifuge?

a)

They move to the outer side

b)

They remain in the middle

c)

They float to the top

14.

What happens to components with higher density when subjected to centrifugal force?

a)

They move outward

b)

They move inward

15.

What is the advantage of separating different components quickly and accurately?

a)

It allows for faster analysis

b)

It is more cost-effective

16.

What should be ensured about the sample tube before centrifugation?

a)

It can withstand centrifugal force

b)

It is clean

17.

What is the purpose of the Alcoholic Lamp or Alcohol Burner?

a)

To provide a stable flame

b)

To heat large containers

18.

What precautions should be taken when using the Alcoholic Lamp?

a)

Use in a well-ventilated area

b)

Keep away from flammable materials

19.

What is the function of the Loop and Needle Set?

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20.

ควรทำอย่างไรหลังจากใช้เครื่องมือเพื่อป้องกันการระเหยของแอลกอฮอล์?

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21.

What should not be done while the lamp is still hot or has fire?

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22.

What is the purpose of the Loop and Needle Set in microbiology?

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23.

What is the first step in using the Inoculating Loop?

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24.

What is the temperature and time for the Denaturation step in PCR?

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25.

What is the temperature range for the Annealing step in PCR?

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26.

อุณหภูมิสำหรับขั้นตอนการขยาย/ยืดใน PCR คืออะไร?

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27.

อุณหภูมิสำหรับขั้นตอนการขยาย/ยืดในกระบวนการสังเคราะห์ DNA คืออะไร?

a)

65°C

b)

72°C

c)

20–40 วินาที

d)

1 นาทีต่อ 1,000 bp

28.

What is the purpose of the pipette and tip set?

a)

To mix solutions

b)

To dispense liquids

c)

To measure temperature

d)

To analyze DNA

29.

What is the function of the Spreader Triangle?

a)

To mix liquids

b)

To spread microbial solutions

c)

To measure DNA

d)

To analyze proteins

30.

What is the purpose of the Vortex Mixer?

a)

To mix liquids rapidly

b)

To measure temperature

c)

To analyze DNA

d)

To dispense liquids

31.

What is a Vortex Mixer?

a)

A device designed to mix liquids in test tubes or flasks by rapid rotation.

b)

A device used to measure the absorbance of light.

c)

A type of microscope.

d)

A device for reading microplates.

32.

What does a Spectrophotometer measure?

a)

The absorbance or transmittance of a substance.

b)

The temperature of a solution.

c)

The pH level of a solution.

d)

The viscosity of a liquid.

33.

What is the principle of operation of a Spectrophotometer?

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34.

ใครเป็นผู้ตั้งชื่อว่า 'กล้องจุลทรรศน์'?

a)

จิโอวานนี ฟาเบอร์

b)

ไอแซค นิวตัน

c)

อัลเบิร์ต ไอน์สไตน์

d)

กาเลลิโอ กาลิเลอี

35.

What does the term 'compound' mean in the context of a compound microscope?

a)

It means the microscope has more than one lens.

b)

It refers to the size of the microscope.

c)

It indicates the microscope is electronic.

d)

It means the microscope is portable.

36.

หากใช้เลนส์ตา 10x กับเลนส์วัตถุ 100x การขยายภาพรวมจะเป็นเท่าไหร่?

a)

1000

b)

100

c)

10

d)

10000

37.

What is the purpose of an inverted microscope?

a)

It is designed for observing specimens from below.

b)

It is used for high magnification.

c)

It is a portable microscope.

d)

It is used for electron microscopy.

38.

What is the function of the objective lens in a microscope?

a)

It is located at the bottom of the stage.

b)

It is used to generate light.

c)

It is used for observing living cells.

d)

It is located at the top of the microscope.

39.

What is the purpose of fluorescence microscopes?

a)

To use fluorescence instead of other light properties.

b)

To create 3D images.

c)

To observe living cells.

d)

To scan the surface of specimens.

40.

เทคนิคที่ใช้ในกล้องจุลทรรศน์แบบคอนฟอคัลคืออะไร?

a)

กล้องจุลทรรศน์แบบสแกนเลเซอร์คอนฟอคัล

b)

การถ่ายภาพฟลูออเรสเซนซ์

c)

กล้องจุลทรรศน์อิเล็กตรอน

d)

กล้องจุลทรรศน์อิเล็กตรอนแบบสแกน

41.

What is the main advantage of scanning electron microscopes?

a)

High-resolution images of surface structures.

b)

Ability to observe living cells.

c)

3D imaging capabilities.

d)

Use of fluorescence.

42.

What is the function of Transmission Electron Microscopes (TEM)?

a)

To create images by detecting electrons

b)

To provide high-resolution images of surface structures

c)

To study the microscopic structure of cells

d)

To visualize the internal structure of specimens

43.

What are the structural components of a microscope?

a)

Head/Body, Base, Arm, Eyepiece, Eyepiece Tube

b)

Lens, Light Source, Stage, Objective Lens

c)

Mirror, Stage Clips, Coarse Focus, Fine Focus

d)

Base, Arm, Stage, Light Source, Eyepiece

44.

What is the purpose of the Eyepiece in a microscope?

a)

To magnify the specimen

b)

To hold the objective lens

c)

To illuminate the specimen

d)

To support the microscope structure

45.

What does the Base of a microscope do?

a)

Supports the structure and houses the light source

b)

Connects the head to the arm

c)

Holds the eyepiece in place

d)

Provides magnification

46.

What is the purpose of the eyepiece tube?

a)

To hold the eyepiece in place

b)

To adjust the focus

c)

To change the objective lens

47.

What is the function of the objective lenses?

a)

To magnify the specimen

b)

To illuminate the specimen

c)

To adjust the focus

48.

What does the nosepiece do?

a)

Holds the objective lenses

b)

Adjusts the focus

c)

Illuminates the specimen

49.

วัตถุประสงค์ของเวทีคืออะไร?

a)

เพื่อถือสไลด์ตัวอย่าง

b)

เพื่อปรับโฟกัส

c)

เพื่อเปลี่ยนเลนส์ตา

50.

What is the function of the aperture?

a)

To control the amount of light

b)

To hold the specimen slide

c)

To adjust the focus

51.

What is the role of the illuminator?

a)

To provide light for viewing the specimen

b)

To magnify the specimen

c)

To hold the objective lenses

52.

What does the condenser do?

a)

Focuses light onto the specimen

b)

Holds the specimen slide

c)

Adjusts the eyepiece

53.

What is the function of the condenser in a microscope?

a)

To focus light onto the specimen

b)

To control the amount of light passing through the specimen

c)

To adjust the position of the condenser lens

d)

To support the microscope

54.

What does the iris diaphragm control?

a)

The amount of light passing through the specimen

b)

The focus of the light

c)

The position of the eyepiece

d)

The support of the microscope

55.

What is the purpose of the eyepiece in a microscope?

a)

To look through at the top of the microscope

b)

To hold the objective lens in place

c)

To support the microscope

d)

To adjust the focus

56.

What is the magnifying power of standard eyepieces?

a)

10x

b)

5x

c)

30x

d)

15x

57.

What does the eyepiece tube do?

a)

Holds the eyepieces in place above the objective lens

b)

Connects to the base of the microscope

c)

Adjusts the focus of the microscope

d)

Supports the microscope head

58.

What is the purpose of the monocular microscope?

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59.

What are the primary optical lenses on a microscope called?

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60.

What is the function of the nosepiece on a microscope?

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61.

What are the Coarse and Fine Focus knobs used for?

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62.

Where is the specimen placed in a microscope?

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63.

What are Stage Clips used for in a microscope?

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64.

What is the purpose of the aperture in a microscope?

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65.

What is the purpose of the stage clips?

a)

To hold the slide in place

b)

To illuminate the specimen

c)

To adjust the focus

d)

To control light intensity

66.

What is the function of the aperture in a microscope?

a)

To allow light to pass through

b)

To magnify the specimen

c)

To focus the light

d)

To hold the slide

67.

What is the role of the illuminator in a microscope?

a)

To provide light

b)

To magnify the specimen

c)

To hold the slide

d)

To adjust the focus

68.

What does the condenser do?

a)

Collects and focuses light

b)

Holds the slide

c)

Magnifies the specimen

d)

Controls light intensity

69.

What is the function of the iris diaphragm?

a)

Controls the amount of light

b)

Holds the slide

c)

Magnifies the specimen

d)

Focuses the light

70.

What does the condenser focus knob do?

a)

Moves the condenser up or down

b)

Controls light intensity

c)

Holds the slide

d)

Magnifies the specimen

71.

What is the purpose of the simple streak technique?

a)

To spread bacteria on the surface of a culture plate

b)

To magnify the specimen

c)

To hold the slide

d)

To control light intensity

72.

What is the purpose of spreading bacteria on the surface of a culture plate?

a)

To distribute bacteria evenly across the plate

b)

To isolate single colonies

c)

To test antibiotic sensitivity

d)

To grow bacteria in a specific pattern

73.

What equipment is used for bacterial streaking?

a)

Inoculating loop

b)

Agar plate

c)

Alcohol lamp

d)

All of the above

74.

What is the first step in the bacterial streaking technique?

a)

Heat the inoculating loop until red hot

b)

Cool the inoculating loop

c)

Inoculate the sample onto the agar plate

d)

Close the lid of the petri dish

75.

What is the purpose of the cross streak technique?

a)

To isolate single colonies

b)

To distribute bacteria evenly

c)

To test for antibiotic resistance

d)

To grow bacteria in a specific pattern

76.

What should be done between each zone in the cross streak technique?

a)

Flame the inoculating loop

b)

Cool the inoculating loop

c)

Inoculate the next zone

d)

Close the petri dish

77.

What are the necessary equipment for collecting samples?

a)

Swab (cotton swab or ready-made swab)

b)

Nutrient agar, MacConkey agar

c)

Alcohol lamp or sterilization equipment

d)

Gloves and protective equipment

78.

What is the basic technique used for collecting bacterial samples?

a)

Swabbing

b)

Dilution

c)

Incubation

d)

Streaking

79.

What is the appropriate temperature for incubating the samples?

a)

30-37°C

b)

20-25°C

c)

40-45°C

d)

10-15°C

80.

What is the purpose of using a spreader in the sample collection process?

a)

To distribute the diluted sample evenly

b)

To sterilize the agar plate

c)

To collect the sample

d)

To measure the temperature

81.

How to use a spreader to distribute the diluted sample evenly on the surface of the agar plate?

a)

Rotate the plate or move the spreader in a systematic direction.

b)

Pour the diluted sample directly onto the agar plate.

c)

Use a pipette to drop the sample onto the plate.

d)

Leave the sample in the center of the plate.

82.

What is the purpose of counting the number of colonies formed after incubation?

a)

To calculate CFU/mL in the original sample.

b)

To determine the type of microorganisms present.

c)

To assess the growth rate of the microorganisms.

d)

To evaluate the effectiveness of the agar medium.

83.

What temperature and time are suitable for incubating the agar plate?

a)

37°C for 24-48 hours.

b)

25°C for 12-24 hours.

c)

30°C for 48-72 hours.

d)

40°C for 6-12 hours.

84.

What is the Spread Plate Method used for?

a)

To isolate and count viable microorganisms in liquid samples.

b)

To prepare agar plates for microbial growth.

c)

To sterilize the agar medium before use.

d)

To measure the pH of the liquid sample.

85.

ใหนิสิตระบุชื่อเรียกเครื่องมือทางวิทยาศาสตรโดยใช้ชื่อเรียก ภาษาไทย แล technical term และบอกหนาที่ของ เครื่องมือดังตอไปนี้ใหถูกตอง

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86.

Machine name

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87.

ชื่อเครื่อง

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88.

หน`าที่/ประโยชนW

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89.

นิสิตเคยมีประสบการณWใช`เครื่องมือ นี้มากNอนหรือไมN

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90.

Have you ever had experience using this tool before?

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91.

Please specify the name of the scientific tool using the Thai name and technical term, and state the function of the tool as follows.

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92.

Have you ever had experience using this tool before?

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93.

Please specify the name of the scientific tool using the Thai name and technical term, and state the function of the tool as follows.

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94.

Have you ever had experience using this tool before?

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95.

Please specify the name of the scientific tool using the Thai name and technical term, and state the function of the tool as follows.

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96.

Have you ever had experience using this tool before?

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97.

Please specify the name of the scientific tool using the Thai name and technical term, and state the function of the tool as follows.

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98.

ใหนิสิตระบุชื่อเรียกเครื่องมือทางวิทยาศาสตรโดยใช้ชื่อเรียก ภาษาไทย แล technical term และบอกหนาที่ของ เครื่องมือดังตอไปนี้ใหถูกตอง

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99.

นิสิตเคยมีประสบการณWใช`เครื่องมือ นี้มากNอนหรือไมN

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100.

Machine name

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101.

What is the role or benefit of the machine?

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102.

Has the student had experience using this tool before?

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103.

Microscopy and Its Application for Diagnosis of Aquatic Diseases

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104.

Fill in the blanks to show the correct components and functions of the microscope.

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105.

Find an example image taken under SEM and TEM, and describe the distinct components found in those images, including clearly stating the source of the images.

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106.

Identify the microbiological techniques used from the following experiments correctly.

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