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WorksheetsBiotechnology Quiz
Total questions: 23
Worksheet time: 12mins
Which organism is the source of Bt toxin gene used in pest-resistant crops?
Rhizobium
Agrobacterium tumefaciens
Bacillus thuringiensis
Pseudomonas
Golden Rice is genetically modified to produce more:
Vitamin D
β-carotene
Vitamin C
Iron
Recombinant human insulin produced using rDNA technology is called:
Novolin
Humulin
Insugen
Protamine
Which technique is used to amplify DNA fragments in molecular diagnosis?
ELISA
PCR
DNA sequencing
Gel electrophoresis
The full form of GEAC is:
Genetic Engineering Appraisal Committee
Genetic Engineering Assessment Council
General Ethical Approval Council
Genetic Evaluation and Advisory Committee
Match the following: Column I (Application) | Column II (Biotechnological Product) a. Emphysema treatment | 1. α-1 antitrypsin b. Nutritional enhancement | 2. Golden rice c. Pest resistance | 3. Bt toxin d. Diabetes treatment | 4. Recombinant insulin
a-1, b-2, c-3, d-4
a-2, b-1, c-4, d-3
a-4, b-3, c-2, d-1
a-3, b-2, c-1, d-4
Which of the following is not an example of biofortification?
Iron-fortified rice
Vitamin A enriched golden rice
Bt cotton
Protein-rich maize
Severe Combined Immunodeficiency (SCID) is caused by the deficiency of:
Adenosine deaminase (ADA)
Insulin
α-1 antitrypsin
Hemoglobin
Which statement about ELISA is incorrect?
It is based on antigen-antibody interaction
It uses enzyme-linked markers
It can detect both antigens and antibodies
It amplifies DNA sequences
A major application of transgenic animals in medicine is:
Increasing milk yield
Production of bioactive products like α-1 antitrypsin
Making crops insect-resistant
Developing drought-tolerant plants
Which of the following crops is genetically modified for pest resistance?
Rice
Bt cotton
Flavr Savr tomato
Golden rice
Biofortification differs from genetic modification because it:
Always increases yield
Improves nutrient quality in crops
Increases resistance to pests
Creates transgenic animals
Choose the correct pair:
ELISA – Amplification of DNA
PCR – Detection of antigen
DNA probe – Complementary base pairing
Bt toxin – β-carotene production
Statement-based: S1: The first GM crop approved for commercial cultivation in India was Bt cotton. S2: Bt cotton provides resistance against bollworm.
Both S1 and S2 correct
S1 correct, S2 incorrect
S1 incorrect, S2 correct
Both incorrect
Bioethics in biotechnology deals with:
Cost of GM crops
Legal rules of patenting
Moral issues of genetic engineering
Hybrid seed production
Which technique is best for early diagnosis of HIV infection?
PCR
ELISA
DNA fingerprinting
Northern blotting
Which enzyme is absent in SCID patients?
Ligase
Adenosine deaminase
DNA polymerase
Restriction enzyme
Identify the correctly matched pair:
Biofortification – pest resistance
Bt cotton – abiotic stress tolerance
Golden rice – provitamin A
SCID – insulin deficiency
GEAC mainly regulates:
Safety of genetically modified organisms
Manufacture of antibiotics
Production of recombinant insulin
Import of hybrid seeds
Which is the correct sequence in PCR?
Denaturation → Annealing → Extension
Extension → Annealing → Denaturation
Annealing → Extension → Denaturation
Denaturation → Extension → Annealing
Match the following techniques with their uses: Technique | Application PCR | Detect antigen/antibody ELISA | DNA amplification DNA probe | Hybridization with complementary sequence
a-1, b-2, c-3
a-2, b-1, c-3
a-3, b-2, c-1
a-2, b-3, c-1
In SCID gene therapy, why are lymphocytes used as target cells?
They can be easily cultured and reintroduced
They contain ADA enzyme naturally
They cannot divide rapidly
They are virus-resistant
Identify the correct statement(s): S1: Biopiracy refers to patenting traditional knowledge or bioresources without consent. S2: Biopatent protects intellectual property of biotechnology inventions.
Only S1 true
Only S2 true
Both S1 and S2 true
Both false
