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WorksheetsUnit 2 Ag biotech
Total questions: 84
Worksheet time: 44mins
Which tool is commonly used in laboratories to determine the amount of liquid in a container accurately?
Beaker
Graduated cylinder
Test tube
Bunsen burner
What is the meniscus?
How do you read a meniscus?
What is the function of a beaker?
To hold, mix, and heat liquids in a laboratory.
What is a common use of an Erlenmeyer flask in laboratory experiments?
To precisely measure the mass of chemicals.
To safely swirl and mix liquids without spilling.
To separate mixtures using a centrifuge.
To observe microorganisms under a microscope.
Which laboratory tool is used to crush solid chemicals into powder form?
Beaker
Mortar and pestle
Test tube
Bunsen burner
Which laboratory equipment is commonly used to grow bacteria in a controlled environment?
Test tube
Petri dish
Beaker
Pipette
Which of the following best describes the use of a weigh boat in a laboratory?
It is used to contain substances while determining their mass on a balance.
It is used to heat samples over a flame.
It is used to filter liquids from solids.
It is used to measure the volume of liquids.
Match the following
Pipette
dispenses small volumes of liquid; > 1mL
Micropipette
moves small volumes; < 1mL
Graduated Cylinder
used for measuring precise volumes
Transfer Pipette
moves small volumes of liquid
What is the primary purpose of using an inoculating loop in microbiology?
To transfer bacteria from one medium to another without contamination.
To observe microorganisms under a microscope.
To measure the pH of a culture medium.
To incubate bacterial cultures at a specific temperature.
Which of the following best describes the primary use of an electric balance in a laboratory?
To measure the volume of liquids accurately.
To determine the mass of substances precisely.
To heat chemicals during experiments.
To observe microscopic organisms.
Why is it important to tare a scale before measuring ingredients?
To ensure the scale is clean before use.
To subtract the weight of the container from the total measurement.
To increase the accuracy of temperature readings.
To measure the volume of liquids accurately.
What is the main function of a magnetic stirrer in a laboratory setting?
To measure the temperature of solutions.
To separate solid particles from liquids.
Ensure efficient and uniform mixing of solutions.
To sterilize laboratory glassware.
Why might a scientist use a water bath during an experiment?
To maintain a constant temperature for samples or reactions.
To measure the pH of solutions
To separate solid and liquid components
To increase the pressure in a reaction vessel
What is the primary function of a spectrophotometer in laboratory experiments?
To determine the pH of solutions
To measure the concentration of substances by analyzing light absorption
To heat samples to a specific temperature
To separate mixtures into their components
What is the primary function of a vortex mixer in a laboratory?
To separate solid particles from liquids.
To mix small amounts of liquid by creating a swirling motion.
To measure the pH of solutions.
To sterilize laboratory equipment.
Which laboratory device is used to rapidly spin small volumes of liquid to separate their components?
Microcentrifuge
Incubator
Vortex mixer
Water bath
Which of the following best describes what happens when a precipitate forms in a chemical reaction?
A solid appears in the solution.
A gas is produced and escapes.
The solution becomes warmer.
The liquid evaporates completely.
What is the primary purpose of using an electrophoresis apparatus in a laboratory?
To sterilize laboratory equipment.
To separate DNA molecules according to their electrical charge and size.
To amplify DNA samples.
To measure the concentration of proteins.
Which laboratory technique commonly uses a thermocycler?
Polymerase Chain Reaction (PCR)
Gel electrophoresis
DNA sequencing
Centrifugation
What is the function of thermocycler?
To facilitate the polymerase chain reaction (PCR) for DNA amplification.
Which of the following best describes the main purpose of PCR in molecular biology?
To make multiple copies of a specific DNA segment.
To synthesize proteins from amino acids.
To separate DNA fragments by size.
To edit genes within living cells.
Why is aseptic technique important in laboratory settings?
It helps to prevent contamination of samples and protects individuals from harmful microorganisms.
It is used to make laboratory equipment more durable.
It increases the speed of laboratory experiments.
It is a method for labeling laboratory samples.
Which of the following are important practices to maintain asepsis?
Washing hands with soap and water
Using sterile instruments
Wearing protective clothing
Sanitizing work surfaces
Leaving equipment uncovered
Abigail is preparing to perform surgery in a hospital. She uses aseptic techniques before starting the procedure. What biological entities is Abigail trying to remove by using these techniques?
Harmful microorganisms (bacteria, viruses, fungi, spores)
Animal tissues
Plant cells
Beneficial bacteria
Which of the following substances can be used to kill harmful microorganisms on surfaces? Choose the correct examples of disinfectants.
Lemon juice and salt
Alcohol (isopropyl alcohol), bleach (sodium hypochlorite), and hydrogen peroxide.
Sugar solution
Olive oil
What is the main reason for minimizing aerosol production during clinical procedures?
It prevents the spread of airborne infectious agents.
It enhances the cleaning power of disinfectants.
Aerosols are beneficial for patient safety.
It increases the efficiency of sterilization equipment.
Which of the following methods are commonly used to sterilize laboratory glassware?
Autoclaving and chemical disinfectants
Drying and freezing
Pasteurization and boiling
Filtration and centrifugation
Which of the following best describes the purpose of media used in laboratories?
To provide nutrients and an environment for microorganisms or cells to grow.
To sterilize laboratory instruments.
To measure the temperature in experiments.
To store laboratory chemicals safely.
Which of the following best describes the primary use of a Laminar Airflow Hood in laboratories?
To maintain a dust-free environment for electronic devices.
To sterilize laboratory glassware using high temperatures.
To provide a sterile working environment by filtering air and preventing contamination.
To store chemicals safely away from direct sunlight.
Which process is commonly performed using an autoclave in laboratories?
Disinfecting surfaces with alcohol.
Sterilizing glassware and instruments with pressurized steam.
Drying laboratory samples.
Measuring the temperature of liquids.
What are the two types of safety symbols?
What does this symbol mean?
What does this symbol mean?
What does the symbol mean?
Harmful to environment with long-lasting effects
Scarlett is trying to examine the tiny details of a leaf that are difficult to see with the naked eye. Which tool should she use?
Hand lens
Ruler
Flashlight
Safety goggles
How are light microscopes different from dissecting microscopes?
What is a key advantage of using electron microscopes over light microscopes?
They are less expensive to operate.
They can magnify objects to a much greater extent.
They are portable and lightweight.
They use visible light for imaging.
Which image was seen with an electron microscope?
Images of landscapes
Images of cells or viruses.
Photos of animals
Pictures of everyday objects
Which of the following best describes the purpose of using a wet mount in microscopy?
To observe living organisms in their natural state using a liquid medium.
To permanently preserve specimens on a slide for future use.
To enhance specimen color by applying a chemical stain.
To magnify specimens without the use of a cover slip.
How do you prepare a bacteria slide?
What is a solution?
What best describes a solute in a solution?
A solute is the substance dissolving the solid.
Which of the following is commonly used to dissolve other substances?
Sand
Water
Iron
Chalk
Which choice is the solvent?
What is a reagent?
Which of the following best describes the function of a buffer in a solution?
It helps maintain a stable pH when acids or bases are added.
It increases the rate of chemical reactions.
It prevents the solution from freezing.
It acts as a source of energy.
Which ion is responsible for the sour taste of acids?
Hydrogen ion (H+)
Potassium ion (K+)
Magnesium ion (Mg2+)
Nitrate ion (NO3-)
What is an acidic solution?
What is a conjugate base?
Formed when an acid loses a proton.
Remains after a base accepts a proton.
Formed when a base gains a proton.
When using a micropipette _____.
depress the plunger to the second stop and release quickly to draw fluid up
hold it horizontally to draw liquid into the internal compartment
push the plunger to the first stop and release slowly to draw fluid up
quickly expel the contents into the center of the microcentrifuge tube
What is a device designed to protect the wearer from inhaling harmful dust, liquid droplets, fumes, vapors, and other materials that may be in the air?
face shield
goggles
lung filter
respirator
Which of the following is a recommended safety practice?
Keep cultures in a sink when not in use.
When possible, pipet broth cultures by mouth.
Mix cultures or reagents in such a way as to avoid splashing.
Wash your hands with cold warm water before leaving the laboratory.
What is this?
Micropipette volumes are accurately measured in:
Centiliters
Liters
Milliliters
Microliters
Agitation of containers of biological entities can result in clouds of liquid particles in the air known as:
Aerosols
Emulsions
Particulates
Pathogens
____ are devices worn or used to protect from injury.
Personal coverage equipment (PCE)
Personal occupational equipment (POE)
Personal protection equipment (PPE)
Personal training equipment (PTE)
Which of the following is an approved practice in the biotechnology laboratory?
Carefully lay test-tubes cultures on the bench top when not in use.
Carry cultures in a large beaker when moving around the laboratory.
Handle all cultures in the laboratory as being potentially pathogenic.
Mop up spilled cultures, and then saturate the area with disinfectant.
What is a laboratory device used for the purpose of thawing substances, speeding reactions, and incubating cultures?
electrophoresis chamber
water bath
vortex mixer
thermocycler
What is a small mass of bacteria on an agar medium that resulted from the reproduction of one cell?
an aggregate
a colony
a growth
a tumor
How can the isolation or separation of bacteria be achieved?
through aseptic technique
through microbial segregation
through serial dilution
through successive filtration
What is essential for viewing individual bacteria?
blue light
oil immersion
methylene blue
Lugol’s solution
What are the procedures used to keep biological materials contained?
aseptic techniques
experimental protocols
microbe-technology
sterilization
What is the purpose of a streak plate?
to isolate bacteria by spreading them across a medium-filled Petri dish
to keep the microorganism cultures at the optimum temperature
to make microorganisms more visible to the naked eye
to show differences in specific types of bacteria
What is the recommended method for sterilizing glassware in an autoclave?
121° C for 20 minutes
121° C for 2 hours
160° C for 20 minutes
160° C for 2 hours
Which is an accepted disinfectant for wiping down a work station?
5% ammonia concentrate
0.5% bleach solution
70% ethanol or isopropyl alcohol
Lysol concentrate is used at 5 percent
What part of a laminar airflow hood is designed to prevent any potentially harmful exposure to the work or the environment?
atmospheric disinfectant
biological entity protectorate
high energy particle air filter
ionic particle purifier
What should be the first step when entering a biotechnology laboratory to conduct work?
Examine containers with biological entities.
Put on a lab coat, safety glasses, and gloves.
Remove any jewelry and wash your hands.
Spray the workstation with a disinfectant
How does an electrophoresis chamber work?
DNA samples are negatively charged and migrate toward the negative terminal based upon size.
DNA samples are positively charged and migrate toward the positive terminal based upon size.
DNA samples are positively charged and migrate toward the negative terminal based upon size.
DNA samples are negatively charged and migrate toward the positive terminal based upon size.
How can a microcentrifuge be balanced if only one sample needs to be spun?
A microcentrifuge tube with water can be placed opposite the sample.
Microcentrifuge tubes should be placed in all the microcentrifuge slots.
The sample should be divided equally between two microcentrifuge tubes.
The microcentrifuge should be operated at half the speed for twice the time.
How often should all work benches and surfaces be decontaminated?
after each activity involving microbial cultures
before leaving an experiment or at least daily
during down time throughout an experiment
when the laboratory is first opened for use
What is an aqueous solution?
A buffer that accepts hydrogen ions.
A solution in which the solvent is water.
A solution that happens to be a gas.
A solution that loses hydrogen ions.
What is the function of an inoculating loop?
to culture cells, grow bacteria, or start plant tissue cultures
to hold the smaller microcentrifuge tubes on laboratory work stations
to inoculate Petri dishes or tubes of growing media by spreading cells
to transfer small amounts of liquids, usually smaller than 5 mL
Which is a tool with a narrow neck used for mixing, stirring, or shaking?
Erlenmeyer flask
graduated cylinder
pipette
weigh boat
What is an illumination technique in which the differences in the phase of light transmitted or reflected by a specimen forms distinct, contrasting images of different parts of the specimen?
bright field microscopy
optical microscopy
phase contrast microscopy
wide field microscopy
What is a combination of either a weak acid and its conjugate base; or a weak base and its conjugate acid?
buffer solution
reagent
solute
solution
What does this HHPS Symbol indicate?
Caution corrosive
Warning corrosive
Danger corrosive
What piece of Personal Protective Equipment is used to protect skin on hands from contact with
biologically hazardous materials?
What should be done to all potentially biologically hazardous materials before disposing of them?
Incinerate all potentially biologically hazardous materials without treatment.
Store potentially biologically hazardous materials indefinitely without action.
Dilute potentially biologically hazardous materials with water before disposal.
Sterilize all potentially biologically hazardous materials before disposal.
What device is used to separate fragments of DNA according to their length?
What term is commonly used to identify the combination of water and nutrients used to grow?
nutrient solution
What technique is used to produce millions of copies of a DNA sequence WITHOUT using
bacteria to amplify the DNA?
