WorksheetsExam 1 review
Total questions: 13
Worksheet time: 10mins
If a bacterial strain expresses a protein that inhibits cleavage of the C5 protein, which of the following processes would be affected?
opsonization
inflammation
membrane attack complex
Which of the following organs lacks afferent lymphatic vessels (vessels where antigens and cells can
use to enter secondary lymphatic organs)?
Adenoids
Peyer's patches
Spleen
Lymph nodes
You decide to generate a single cell suspension from organ X. After obtaining your suspension, you
decide to count the cells using the trypan blue exclusion method and after counting the first two
quadrants, you get an average of 85 live cells per square. What should you do?
dilute the sample less, re-load in the
hemacytometer and recount
dilute the sample more, re-load in the
hemacytometer and recount
keep counting all 8 quadrants
repeat the entire isolation starting from the
actual organ
You are trying to identify a potential vaccine candidate by testing which Salmonella antigens can induce the generation of antibodies in infected patients via western blot. Specifically, you separate five Salmonella antigens (see table) on a protein gel, transfer them to a membrane and incubate them with serum from infected patients. Based on the obtained results, which antigen is the best candidate for vaccine development?
Antigen 1
Antigen 2
Antigen 3
Antigen 4
Antigen 5
In which component of a report would you find the reasoning (the why) behind the conducted experiments?
Introduction
Materials and methods
Results
Discussion
You decide to perform a trypan blue exclusion assay to determine the concentration and viability of your single cell suspension. To count your cells, you first mix 30 µL of the isolated cells with 90 µL of PBS, followed by mixing 20 µL of the first dilution with 20 µL of Trypan blue. What is the final dilution factor you used?
1/2
1/4
1/6
1/8
Which of the following would protect a scientist from getting their eyes splashed with chemicals?
Lab coat
gloves
safety googles
respirator
Based on the obtained results, which pathway is more likely activated in the presence of this bacterial species?
Alternative
Classical
Lectin
You and your lab mate perform a western blot to detect protein X (expected size: 85 kDa) and obtained the following results. What are some possible reasons as to why does your membrane looks like this? (Select all that apply)
air bubbles trapped between the membrane and gel
the concentration of the 2ary Ab is too high
protein sample overloading
the concentration of the 1ary Ab is too high
You decide to generate a single cell suspension from organ X. After obtaining your suspension, you decide to count the cells using the trypan blue exclusion method and after counting the first two quadrants, you get an average of 8 live cells and 30 dead cells per square. What should you do?
keep counting all 8 quadrants
repeat the entire isolation starting from the actual organ
dilute the sample more, re-load in the hemacytometer and recount
dilute the sample less, re-load in the hemacytometer and recount
___________ is the place of where B cells become activated. (Select all that apply)
lymph nodes
bone marrow
adenoids
thymus
If you want to replicate a scientist's experiment, which component of the report would you use?
Introduction
Materials and methods
Results
Discussion
Which of the following would mainly protect a scientist from aerosolized particles getting into the respiratory tract?
lab coat
gloves
safety googles
respirator
