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WorksheetsReticulocytes and Supravital Staining Principles Worksheet
Total questions: 15
Worksheet time: 5mins
Reticulocytes are immature red blood cells that still contain:
Nucleus
Ribosomal RNA
Hemoglobin precursor granules
DNA fragments
The reticulocyte count provides an estimate of:
Red blood cell lifespan
Rate of erythropoietin release
Rate of red blood cell production in the bone marrow
Percentage of nucleated red cells in circulation
The presence of increased reticulocytes in peripheral blood is termed:
Reticulocytopenia
Reticulocytosis
Polychromasia
Basophilia
The normal reference range for reticulocytes in adults is approximately:
0.1 – 0.5%
0.5 – 2.0%
2 – 5%
5 – 10%
Supravital stains are dyes that stain:
Fixed and dead cells
Living cells
Only red blood cells
Only white blood cells
The most commonly used supravital stain for reticulocyte counting is:
Wright stain
Hematoxylin
New Methylene Blue
Prussian Blue
When stained with New Methylene Blue, reticulocytes appear as cells containing:
Purple nuclei
Blue cytoplasmic granules
Dark-blue reticular or granular network
Pink homogeneous cytoplasm
Another supravital dye that can be used for reticulocyte staining besides New Methylene Blue is:
Eosin Y
Brilliant Cresyl Blue
Giemsa
Leishman
The incubation time for the reticulocyte staining mixture (blood + stain) is typically:
A. 1–2 minutes at room temperature
B. 10–15 minutes at 37°C
C. 30–45 minutes at 4°C
D. Overnight at room temperature
When performing a manual reticulocyte count, the number of reticulocytes is counted among:
100 WBCs
500 RBCs
1,000 RBCs
All cells in five fields
The formula for the percentage reticulocyte count is:
(Reticulocytes counted / RBCs counted) × 100
(RBCs counted / Reticulocytes counted) × 100
(Reticulocytes counted × RBC count) ÷ 100
(RBCs counted / WBCs counted) × 100
The corrected reticulocyte count accounts for:
Patient’s plasma volume
Patient’s hematocrit
Patient’s MCV
WBC count
The formula for corrected reticulocyte count is:
Retic % × (Patient’s Hct / 45)
Retic % × (45 / Patient’s Hct)
In anemia with an inappropriately low corrected reticulocyte count, the problem is most likely due to:
Blood loss
Hemolysis
Decreased bone marrow production
Increased erythropoietin response
The Reticulocyte Production Index (RPI) is used to:
Correct for hematocrit and maturation time
Measure plasma hemoglobin
Identify reticulocyte shape abnormalities
Calculate absolute reticulocyte number only
