WorksheetsHEMA-12
Total questions: 58
Worksheet time: 29mins
Name
Class
Date
1.
What type of hemocytometer is commonly used for manual cell counts?
a)
Petroff-Hausser chamber
b)
Fuchs-Rosenthal chamber
c)
Improved Neubauer chamber
d)
Bürker-Türk chamber
2.
How many identical sides does the Improved Neubauer counting chamber have?
a)
1
b)
2
c)
3
d)
4
3.
What is the total area of the Improved Neubauer counting chamber?
a)
1 mm × 1 mm
b)
2 mm × 2 mm
c)
3 mm × 3 mm
d)
4 mm × 4 mm
4.
How many large squares is the counting chamber divided into?
a)
4
b)
9
c)
16
d)
25
5.
The four large corner squares of the Improved Neubauer chamber are used for counting:
a)
RBCs
b)
Platelets
c)
WBCs
d)
Reticulocytes
6.
The large corner squares used for WBC counting are subdivided into how many smaller squares each?
a)
9
b)
16
c)
25
d)
36
7.
How many smaller squares is the central large square (used for RBC counting) divided into?
a)
9
b)
16
c)
25
d)
36
8.
The central large square of the Improved Neubauer chamber is used for counting:
a)
WBCs
b)
RBCs
c)
Platelets
d)
Reticulocytes
9.
How long should the diluted blood be allowed to stand to ensure RBC lysis before counting WBCs?
a)
2 minutes
b)
5 minutes
c)
10 minutes
d)
30 minutes
10.
Within how many hours should WBC counts be performed after dilution?
a)
1 hour
b)
2 hours
c)
3 hours
d)
6 hours
11.
What is the purpose of placing the hemocytometer in a moist chamber before counting?
a)
To dry the sample faster
b)
To stain the cells evenly
c)
To allow the cells to settle without evaporation
d)
To sterilize the chamber
12.
What is the typical dilution used in a manual WBC count?
a)
1:10
b)
1:50
c)
1:100
d)
1:20
13.
At what angle should the microhematocrit tube be held when charging the hemocytometer?
a)
30 degrees
b)
45 degrees
c)
60 degrees
d)
90 degrees
14.
How long should the hemocytometer remain in a moist chamber before counting the cells?
a)
5 minutes
b)
10 minutes
c)
15 minutes
d)
20 minutes
15.
Where should the tip of the microhematocrit tube touch when charging the hemocytometer?
a)
The center of the coverslip
b)
The edge of the chamber away from the coverslip
c)
The coverslip edge where it meets the chamber floor
d)
The middle of the counting grid
16.
Why should both sides of the hemocytometer be charged?
a)
To allow duplicate counts for better accuracy
b)
To prevent air bubbles from forming
c)
To balance the liquid pressure inside the chamber
d)
To speed up the counting process
17.
What does a shift to the left indicate in a peripheral blood smear?
a)
a. Increase in the number of old neutrophil forms
b)
b. Increase in the number of young neutrophil forms
c)
c. Decrease in WBC count only
d)
d. Presence of teardrop RBCs
18.
In which type of shift to the left is there an increase in young neutrophil forms but a normal or decreased WBC count?
a)
a. Regenerative shift to the left
b)
b. Degenerative shift to the left
c)
c. Shift to the right
d)
d. Leukoerythroblastic reaction
19.
Appendicitis is an example of which type of shift?
a)
a. Regenerative shift to the left
b)
b. Degenerative shift to the left
c)
c. Shift to the right
d)
d. Leukoerythroblastic reaction
20.
Tuberculosis is associated with which type of shift?
a)
a. Regenerative shift to the left
b)
b. Degenerative shift to the left
c)
c. Shift to the right
d)
d. Leukoerythroblastic reaction
21.
A shift to the right is characterized by which finding?
a)
a. Increase in the number of young forms
b)
b. Increase in the number of old neutrophil forms
c)
c. Increase in immature RBCs
d)
d. Decrease in eosinophils
22.
The presence of immature neutrophils, nucleated RBCs, and teardrop RBCs in a peripheral smear is indicative of:
a)
a. Regenerative shift to the left
b)
b. Degenerative shift to the left
c)
c. Leukoerythroblastic reaction
d)
d. Shift to the right
23.
Leukoerythroblastic reaction is strongly associated with which condition?
a)
a. Acute leukemia
b)
b. Primary myelofibrosis (PMF)
c)
c. Iron deficiency anemia
d)
d. Megaloblastic anemia
24.
What is the color of the bead inside the RBC Thoma pipet?
a)
Blue
b)
White
c)
Red
d)
Colorless
25.
What is the color of the bead inside the WBC Thoma pipet?
a)
Red
b)
Blue
c)
White or colorless
d)
Green
26.
What are the graduation markings on the RBC Thoma pipet?
a)
a. 0.1, 0.5, and 11
b)
b. 0.5, 1, and 101
c)
c. 0.5, 10, and 100
d)
d. 0.1, 1, and 10
27.
What are the graduation markings on the WBC Thoma pipet?
a)
a. 0.5, 1, and 11
b)
b. 0.5, 1, and 101
c)
c. 0.5, 10, and 100
d)
d. 0.1, 1, and 10
28.
What is the total volume capacity of the bulb in an RBC Thoma pipet?
a)
Option 1
b)
10
c)
11
d)
100
e)
101
29.
What is the total volume capacity of the bulb in a WBC Thoma pipet?
a)
Option 1
b)
10
c)
11
d)
100
e)
101
30.
Which hematology analyzer uses the VCS technology (Volume, Conductivity, Scatter) for WBC differential counting?
a)
a. Sysmex XN Series
b)
b. Siemens ADVIA 2120i
c)
c. Beckman Coulter Unicel DxH 800
d)
d. Abbott CELL-DYN Sapphire
31.
The Sysmex XN Series determines hemoglobin concentration using which method?
a)
a. Modified cyanmethemoglobin
b)
b. Sodium lauryl sulfate method
c)
c. Azide methemoglobin method
d)
d. Cyanide-free alkaline hematin method
32.
In the Abbott CELL-DYN Sapphire, the WBC differential count is based on which principle?
a)
a. Impedance and conductivity
b)
b. Peroxidase staining and light scatter
c)
c. Multia-angle polarized scatter separation (MAPSS) and fluorescence detection
d)
d. Fluorescent staining and side scatter
33.
Which instrument measures hemoglobin at 546 nm using the modified cyanmethemoglobin method?
a)
a. Beckman Coulter Unicel DxH 800
b)
b. Sysmex XN Series
c)
c. Abbott CELL-DYN Sapphire
d)
d. Siemens ADVIA 2120i
34.
The hematocrit in most automated hematology analyzers (except Sysmex) is calculated using which formula?
a)
a. (MCV × RBC) / 10
b)
b. (RBC × RDW) / 100
c)
c. (Hgb × 3) / 100
d)
d. (MCV × WBC) / 10
35.
Which hematology analyzer determines hematocrit by cumulative RBC pulse height detection?
a)
a. Beckman Coulter Unicel DxH 800
b)
b. Sysmex XN Series
c)
c. Abbott CELL-DYN Sapphire
d)
d. Siemens ADVIA 2120i
36.
The Siemens ADVIA 2120i uses peroxidase staining and laser light scatter for:
a)
a. RBC indices determination
b)
b. Reticulocyte count
c)
c. WBC differential
d)
d. NRBC enumeration
37.
In hematology analyzers, what does VCS stand for, and what is it used for?
a)
a. Volume, Conductivity, Scatter — used for WBC differential counting in Beckman Coulter analyzers
b)
b. Voltage, Capacitance, Sensitivity — used for RBC volume measurement
c)
c. Volume, Conductivity, Sensitivity — used for reticulocyte detection in Sysmex analyzers
d)
d. Voltage, Current, Scatter — used for platelet counting in Abbott analyzers
38.
What does MAPSS stand for in hematology instrumentation?
a)
a. Multi-Angle Polarized Scatter Separation — used for WBC differential in Abbott CELL-DYN analyzers
b)
b. Multi-Angle Photon Scatter Spectrum — used for RBC morphology in Siemens analyzers
c)
c. Monochromatic Absorption Particle Scanning System — used for platelet count in Beckman analyzers
d)
d. Multiple Axis Phase Scatter Signal — used for reticulocyte analysis in Sysmex analyzers
39.
Which analyzer utilizes VCS technology for differentiating WBCs based on size, internal complexity, and light scatter?
a)
a. Siemens ADVIA 2120i
b)
b. Beckman Coulter Unicel DxH 800
c)
c. Sysmex XN Series
d)
d. Abbott CELL-DYN Sapphire
40.
Which analyzer employs the MAPSS (Multi-Angle Polarized Scatter Separation) technology for WBC differential?
a)
a. Sysmex XN Series
b)
b. Beckman Coulter Unicel DxH 800
c)
c. Abbott CELL-DYN Sapphire
d)
d. Siemens ADVIA 2120i
41.
-nuclear remnants of lymphocytes -appearance similar to a thumbprint
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
42.
may be associated with chronic lymphocytic leukemia (CLL)
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
43.
may be found normally in few numbers
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
44.
-structureless chromatin
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
45.
nuclear remnants of granulocytic cells
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
46.
granulocytic cells with pyknotic nuclei and an agranular cytoplasm
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
47.
-prolonged exposure to EDTA -chemotherapy (rarely)
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
48.
-overwhelming septicemia -bacterial and fungal infections
-erythrophagocytosis (in which neutrophil engulfs RBCs)
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
49.
-neutrophils that have engulfed foreign bodies like bacteria and fungi
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
50.
netlike chromatin pattern
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
51.
may be seen in some leukemias
a)
Smudge cells
b)
Basket cells
c)
Necrotic cells
d)
Phagocytic cells
52.
-large cells (20 to 30 um) which line the veins
a)
Endothelial cells
b)
Megakaryocyte fragments
c)
Nucleated red blood cells
53.
stretched and ovoid in appearance -have a single nucleus with dense chromatin and no nucleoli
-abundant cytoplasm which appears translucent
-usually found at feather edge
a)
Endothelial cells
b)
Megakaryocyte fragments
c)
Nucleated red blood cells
54.
-considered a contaminant of venipuncture (found occasionally in blood obtained by venipuncture) and must not be confused with clumps of malignant cells
a)
Endothelial cells
b)
Megakaryocyte fragments
c)
Nucleated red blood cells
55.
-nude nuclei that stain dark purple
a)
Endothelial cells
b)
Megakaryocyte fragments
c)
Nucleated red blood cells
56.
-found in newborns
-may be found also in aberrant platelet production, myelofibrosis, essential thrombocythemia
a)
Endothelial cells
b)
Megakaryocyte fragments
c)
Nucleated red blood cells
57.
-varies according to cell maturity
a)
Endothelial cells
b)
Megakaryocyte fragments
c)
Nucleated red blood cells
58.
-found in newborns
-may be found also in cases of hemolysis, leukemia, myeloproliferative disorders, and others
a)
Endothelial cells
b)
Megakaryocyte fragments
c)
Nucleated red blood cells
100 %
