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Bordetella Cultural, Biochemical, and General Characteristics

Total questions: 11

Worksheet time: 6mins

Name
Class
Date
1.

Which media is Bordetella pertussis commonly cultured on?

a)
MacConkey agar
b)
Chocolate agar
c)
Nutrient agar
d)
Bordet-Gengou agar
2.

When Bordetella pertussis is grown on selective media, its colonies are typically described as:

a)

Large, flat, and spreading, with a metallic sheen.

b)

Smooth, glistening, small pearly grey shiny (mercury-like), and mucoid.

c)

Dry, wrinkled, and pigmented deep purple.

d)

Rapidly growing, hemolytic, and rough.

3.

How does the growth of Bordetella parapertussis differ from that of Bordetella pertussis?

a)

B. parapertussis is non-hemolytic, while B. pertussis is hemolytic.

b)

B. parapertussis grows more slowly and forms smaller colonies.

c)

B. parapertussis grows more rapidly and forms larger colonies.

d)

B. parapertussis only grows anaerobically.

4.

Which key biochemical test result is characteristic of Bordetella pertussis but is negative for B. parapertussis and B. bronchiseptica?

a)

Oxidase positive

b)

Urease positive

c)

Motility positive

d)

Citrate positive

5.

Which Bordetella species is characteristically positive for both Urease and Motility?

a)

Bordetella pertussis

b)

Bordetella parapertussis

c)

Bordetella bronchiseptica

d)

Bordetella holmesii

6.

In a Gram stain, Bordetella pertussis is described as a small, non-motile organism with the shape of a:

a)

Gram-positive rod

b)

Gram-negative coccobacillus

c)

Gram-negative spirochete

d)

Gram-positive coccus in chains

7.

Bordetella organisms may be arranged in which formation when viewed under the microscope?

a)

Large clumps or clusters only

b)

Singly or in pairs

c)

Pleomorphic, palisade arrangements

d)

Tetrads or sarcinae

8.

During which of the three recognized stages of Bordetella pertussis infection is the patient considered most highly contagious and likely to transmit the bacterium?

a)
Catarrhal stage
b)
Incubation period
c)
Paroxysmal stage
d)
Post-cough stage
9.

Which virulence factor is believed to allow Bordetella pertussis to attach firmly to the ciliated epithelial cells of the respiratory tract?

a)
Adhesion protein A
b)
Filamentous hemagglutinin (FHA)
c)
Ciliated epithelial toxin
d)
Respiratory surface factor
10.

Protection against whooping cough is included in which vaccine given to infants at 2, 4, and 6 months?

a)
DTaP vaccine
b)
PCV vaccine
c)
Hib vaccine
d)
MMR vaccine
11.

What is the preferred specimen type for the laboratory isolation of Bordetella pertussis?

a)
Throat swab
b)
Sputum sample
c)
Nasopharyngeal swab
d)
Blood culture