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WorksheetsPOST TEST MIKRO 1.4
Total questions: 10
Worksheet time: 5mins
Gram stain is the most commonly used staining method for microscopic examination of bacteria. What is the stain category of Gram staining?
Simple staining
Differential staining
Capsule staining
Spore staining
Negative staining
Examples of staining methods that can be used to stain bacterial capsules are:
Gray's method
Gins-Burri's method
Albert's method
Klein's method
Gram's method
In Gram staining, the part of the cell wall responsible for the color difference between gram-positive and gram-negative bacteria is:
Lipopolysaccharide found in gram-positive bacteria
Peptidoglycan in gram-negative bacteria is thicker than in gram-positive bacteria
Peptidoglycan in gram-positive bacteria is thicker than in gram-negative bacteria
Lipopolysaccharide found in gram-negative bacteria
Mycolic acid found in gram-positive bacteria
In the gram staining method, the reagent that produces a red/pink color on the bacterial wall is:
Methylene blue
Carbol fuchsin
Safranin
Crystal violet
Toluidine blue
In Gram staining, gram-positive bacteria will appear as:
blue
red
purple
green
brown
The function of Lugol's reagent in Gram staining is:
It is the first reagent used and stains all cells purple.
It increases the affinity of cells for the primary dye and forms an insoluble crystal violet-iodine (CV-I) complex.
It acts as a protein dehydrator and lipid solvent.
It stains bacterial cells red/pink.
It stains bacterial cells brown.
An example of gram-negative coccus bacteria in gram staining is:
Streptococcus pneumoniae
Lactobacillus acidophilus
Escherichia coli
Neisseria gonorrhoeae
Staphylococcus aureus
Examples of gram-positive rod-shaped bacteria in gram staining is:
Streptococcus pneumoniae
Lactobacillus acidophilus
Escherichia coli
Neisseria gonorrhoeae
Vibrio cholerae
The correct reagents for Gram staining, based on the sequence of procedures, are:
Crystal violet → Lugol → Ethyl alcohol → Safranin
Safranin → Lugol → Ethyl alcohol → Crystal violet
Crystal violet → Lugol → Ethyl alcohol → Carbol fuchsin
Toluidine blue → Lugol → Ethyl alcohol → Carbol fuchsin
Safranin → Lugol → Ethyl alcohol → Toluidine blue
Examples of bacteria that have terminal spores is:
Bacillus subtilis
Bacillus anthracis
Corynebacterium diphtheriae
Proteus mirabilis
Clostridium tetani
