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WorksheetsMock First Professional Examination
Total questions: 122
Worksheet time: 1hrs 1mins
Wet preparation can be observed with these objectives.
×4
×10
×40
×100
×400
Immersion oil can be removed from objectives lens with.
xylene
methylated spirit
soft cloth
lens paper
Glycerol
----- specimen can be sent for bacteriological culture in an unsterile clean container.
Blood
Cerebrospinal fluid
urine
sputum
stool
Wet preparation is used to examine.
specimens and cultures for motile bacteria
specimens for cell type
CSF for capsulated yeast cells
stool samples for ova and parasites
morphological arrangement of bacteria
Examination of urine deposit reveal the presence of.
calcium oxalate
Red blood cells
white blood cells
spermatozoa
kidney stones
These are Gram negative organisms.
Neisseria
Staphylococci
Streptococci
Klebsiella
Proteus
Simple bacteriological stains includes.
Crystal violet
methylene blue
carbol fuchsin
copper sulphate
Indian ink
Ziehl Neelsen staining procedure for sputum AFB is.
Carbol fuchsin, rinse, acid alcohol rinse, malachite green.
Strong Carbol fuchsin with mild heating, rinse, 3% acid alcohol, rinse, malachite green
Strong Carbol fuchsin with mild heating, rinse, 3% acid alcohol, rinse, methylene blue
Strong Carbol fuchsin with mild heating, rinse, 10% acid alcohol, rinse, methylene blue
Strong methylene blue with mild heating, rinse, 3% acid alcohol, rinse, Carbol fuchsin
Preparation of chocolate agar media involve.
use of 10% blood agar
Heating blood agar mixture in water bath at 100°C until turn chocolate
Heating blood agar mixture in a water bath at 70 - 80°C until turn chocolate
use of 4% blood agar
Heating blood agar mixture in autoclave until turn chocolate
Choice media for urine culture are.
Blood agar
chocolate agar
CLED agar
nutrient agar
Saboraud agar
Choice media for stool culture are.
CLED agar
DCA
Blood agar
nutrient agar
selenite F
MacConkey agar is sterilised using.
Sieze filter
Autoclave
Hot air oven
Lysol
Tyndalisation
Escherichia coli is.
motile
urease positive
citrate positive
indole negative
lactose fermenting
Staphylococcus aureus is.
motile
Gram negative cocci
catalase positive
coagulase positive
appears in chains
Psuodomonas aeruginosa is.
Gram negative bacilli
motile
oxidase positive
strict aerobe
a non-lactose fermenter
Bacterial growth in blood culture is evidenced by.
floccular growth on top of medium
Haemolysis
increased volume of broth
Turbidity of the medium
All of the above
MacConkey agar can be use for the isolation of.
Neisseria gonorrhoeae
Streptococcus viridian
Corynebacterium dipthera
Mycobacterium bovis
Proteus mirabilis
Anaerobic cultivation is achieved using.
Blood agar in CO2 jar
Glucose deep agar
iron stripe method
Double plate method
All of the above
Vibio cholera is selectively grown on.
50% Crystal violet blood agar
manatol salt agar
Thiosulphate citrate bile salt agar
Alkaline peptone water
----- organism is positive for oxidase test.
Neisseria gonorrhoeae
Streptococcus viridians
Haemophilus influenza
Mycobacterium bovis
Proteus mirabilis
Exposure to fume of formalin brings about this condition.
conjunctivitis
Nasal irritation
Headache
ill health
Tonsillitis
The application of 20% alcohol brings about the.
spreading out of section
Hely's bond
proper fixation
staining
Good dehydration
The stain used demonstrating epithelial cells in non-gynaecological samples includes.
papanicolaou
ziehl Neelsen
Haematoxylin and eosin
Romanowsky stain
Verhoeff-vanGieson
The following is a common sectioning tool in histopathology laboratory.
Carmel hair brush
Tissue processor
Petri dish
Couplin jar
20% alcohol
In honing the following movement is observed.
Back of knife leads
cutting lacet upwards
oscillation
Back of knife trails
cutting facet leads
In stropping the following movement is observed.
Oscillation
Back of knife leads
Back of knife upwards
cutting facet leads
cutting facet frails
The differentiation of section in Haematoxylin and eosin staining technique is enhanced by.
water
1% acid alcohol
1% alcohol
10% acid alcohol
10% alcohol
The temperature of water bath used for sectioning a tissue embedded in soft wax is.
10-15°C
40-45°C
50-55°C
60-65°C
35-40°C
Canada basalm is miscible with this solvent.
Toulene
Xylene
DPX
cedarwood oil
liquid paraffin wax
Sections are for microscopic examination at.
3-5mm thickness
3-5u thickness
5-8mm thickness
5-8u thickness
4-6cm thickness
Hydration of section during staining enhances the.
uptake of stains
uptake of cleaning agents
uptake of alcohol
uptake of cedar wood oil
uptake of mountant
In preparing 10% formol saline, the inclusion of this constituent is essential.
sodium chloride
potassium dichromate
Alcohol
potassium hydroxide
Aluminum oxide
This tool enables the attachment of tissue block to wooden block.
molten wax
the spatula
Heat
microtome
mountant
Honing of microtome knife could be done on.
Belgian yellow stone
leather sheep
Indian yellow stone
American granite stone
Soweto yellow stone
The decalcifying agent used for surface decalcification in embedded tissue is.
10% hydrogen peroxide
10% sulphuric acid
mollifex solution
concentrated ammonia
The most common adhesive agent used in cytology is.
Albumin
yolk
molten wax
sodium chloride
Alcohol
Which of these materials is used in the collection of cervical smear.
frosted end Slide
cryostat
facemask
eosin solution
Applicator stick
Weigert Haematoxylin is used in one of the following ways.
solution A and B are used immediately after preparation
solution A and B are mixed shortly before use
solution B is used first and then A later.
solution A is never mixed with B
solution A and B used as counter stains
Testicular biopsy is usually preserved in.
Mercury solution
Ethanol
formaldehyde
potassium dichromate
Bouin's fluid
The staining time for Erlich Haematoxylin is.
half of an hour
15 mins
three quarter of an hour
1 hour
Briefly
Iodine preparation is used mainly to identify.
segments of parasite
Adult
Cyst
Larvae
Schultner's dot
Which of these parasites is found in a skin snip sample.
Onchocerca vovulus
Paragonimus westermani
Leishmania
Trypanosoma species
Toxoplasma gondii
In collecting specimen for parasitological investigation, the specimen container must possess the following qualities except.
leak proof
clean
Dry
Free from antiseptic and disinfectant
Heparinized container
r must possess the following qualities except.
leak proof
clean
Dry
Free from antiseptic and disinfectant
Heparinized container
The egg of one of these parasites has a terminal spine.
Shistosoma haematobium
Shistosoma japanicum
Shistosoma mansoni
Shistosoma makongi
Which of these is one of the reagents used for formol ether concentration technique.
iodine
Diethyl ether
Distilled water
Dimethyl ether
Diethyl alcohol
One if these is a layer formed in formol ether concentration after centrifugation.
Faecal debris
water
Diethyl and dissolved glucose
Faecal Buffy
Kupfer layer
Which of these is not a non-parasitic structure found in faeces.
Charcol leydon crystals
Starch cell
proglotides
yeast
spores
The presence of ingested cells in the cytoplasm is a diagnosis feature for this parasite.
Fasciola hepatica
Entamoeba histolytica
Tania species
Shistosoma haematobium
Shistosoma mansoni
The type of blood film used for speciation of plasmodium is.
Thick blood film
thin blood film
Blood smear
whole blood
Blood serum
The major constituent of Giemsa stain is.
azure and eosin
Eosin and alcohol
Eosin and methyl green
Eosin and saffranin
Eosin and water
Malaria parasites in thick and thin blood film require staining pH.
6.1-6.2
7.1-7.2
8.1-8.2
5.1-5.2
9-10
Which of these objectives lenses is best employed for stained films.
immersion oil lens
×100
×40
×250
×10
This stage of Plasmodium can be found in blood film except.
larvae
Cyst
Gametocytes
merozoites
Ova
The microscopic examination of scanty parasites in a blood specimen is best processed using.
centrifuged blood
thin film
wet preparation
thick film
serum smear
The commonest cause for not finding parasites in patients with malaria is.
malaria pigment
Too few parasites in peripheral blood
Effect of alcohol on red blood cells.
Excess immersion oil
Over differentiation
The blood film made for malaria parasites investigation is dried on a hot plate for.
60 minutes
30 minutes
15 minutes
10 minutes
5 minutes
The ova of Trichuris trichuira has a unique shape referred to as.
Barrel
mutoid
Elliptical
Spindle
Oval
The fertilised ovum of Ascaris lumbricoides has a characteristics pattern.
Uneven albuminous coat
Even albuminous coat
Large cylindrical coat
Bulbilous coat
Yellowish coat
The ovum of hookworm is referred to as.
Round
oval and segmented
cylindrical and segmented
Barrel and unsegmented
Rod-like
The shape of Entamoeba histolytica is.
Regular
Irregular
Cylindrical
Oval
Spindle
Which is true about the AUTO-CONTROL in ABO Blood grouping.
It helps to detect anti-M antibodies
It helps to detect anti-N
It helps to detect auto- immune antibodies
It helps to detect anti-A antibodies
What is critical in the grouping of cord blood.
Through washing of the red blood
Warming of the red blood cells
mixing of the red blood cells
Spinning of the blood cells
None of the above
The positive control in direct comb's test is same as.
Weakly sensitized A The D positive cells
Weakly sensitized O Rh D positive cells
Weakly sensitized B Rh D positive cells
Weakly sensitized AB Rh D positive cells
Strongly sensitized O Rh D positive cells
Washing of cord blood ensures.
Complete removal of Wharton's jelly
To include Wharton's jelly
To make use of the Wharton's jelly
To prepare the cord cells for transportation
None of the above
The use of A2 cells in ABO Blood grouping helps to detect.
potency of Anti-A sensitized plasma
potency of Anti-A sensitized complement
potency of sensitized antibodies
potency of Anti-A
potency of red cells coated with platelets
What does indirect comb's test detect?
IgM antibodies
ABO and Rhesus antigen
Anti-A and Anti-B
incomplete IgG antibodies
platelets coated antibodies
The DCT can be used for.
Compatibility testing
Blood grouping
Detecting sensitized red cells.
Detecting Haemolysed red cells
None of the above
The ICT can be used for.
Blood grouping
Total red cell count
Compatibility testing
Total white cell count
None of the above
Which of these anticoagulant is used for blood donor collection?
Acid citrate dextrose
EDTA
Heparin
Flouride
Non-heparinised containers
Ante-natal screening and serology is to detect.
Tissue antigen
Tissue antibodies
Immune antibodies in the system of pregnant women
HLA antigens
None of the above
Compatibility testing is the same as.
Cross-labelling of blood
cross-tagging of units of blood
screening of units of blood
cross-matching of blood
cross-tagging of donor and recipient blood
Bovine albumin is used in cross matching to ensure.
Better antigen and complement association
More sensitive antigen- antibody reactions
Plasma and protein association
serum and hapten associations
All of the above
Red blood cells suspended in low ionic strength saline.
React in same way as in normal saline
React less than in normal saline
React better than in normal saline
Not react at all
React as in (a) and (b) above
Transfusion of platelet concentrate helps to.
Correct anaemia
Correct loss of platelets
Correct loss of leucocytes
Correct bleeding disorders
Correct polycythemia
Blood component transfusion means.
Giving the part of blood that is most beneficial to the patient
Giving whole blood
Giving whole blood and plasma
Giving whole blood and platelets
None of the above
The anticoagulant for coagulation studies is.
Acid citrate dextrose
sodium citrate
calcium oxalate
potassium Flouride
Which is TRUE about IgM antibodies?
They are incomplete antibodies
They are complete antibodies
They are correct antibodies
They are incorrect antibodies
They are cold reacting antibodies
The Erythrocyte Sedimentation Rate (ESR) is a.
Test for vascular integrity
Test for infections only
A non-specific test but helps in diagnosis of certain diseases
Test for cancer only
A specific test that helps in the diagnosis of certain diseases
Which is true for IgM agglutinins.
They react best at 37°C
They react best at 27°C
They react best at 4°C
They react weakly at 2°C
They react poorly at 6°C
Blood collected in HEPARIN anticoagulant is not suitable for film staining because it will.
Haemolyse the red cells
It will stain the background of the slide and render vision blur
It will Haemolyse the white cell and make the vision difficult
It will haemolyse the platelet and render vision difficult
It will stain the background of the slide and render vision easy
Blood substitutes are not.
Artificial blood surrogates
Used to mimic the functions of biological blood
Oxygen carriers
Perfluorocarbons
Biological blood components
Patients with low PCV and haemoglobin levels will be transfused with.
packed red cells (PRBC)
Platelet concentrate (PLTC)
Leucocyte concentra
Patients with low PCV and haemoglobin levels will be transfused with
packed red cells (PRBC)
Platelet concentrate (PLTC)
Leucocyte concentrate
Fresh frozen plasma (FFP)
only b and c above
The microhaematocrit centrifuge is programmed to function at
5000rpm for 5 minutes
10,000rpm for 5 minutes
20,000rpm for 10 minutes
30,000rpm for 10 minutes
All of the above
The Neubauer counting chamber is used for:
Counting money
Counting test tubes
Counting sample bottles
Cell enumeration
Assisting the ATM machine
The westergren pippette is used for
Measurement of red blood cells only
measurement of white blood cells only
Measurement of platelets and red blood cells
ESR estimation
ESR estimation and PCV
Red cell antigens are found:
On the red cell membrane
On the white cell coat
in the Platelet matrix
Inside the red blood cell matrix
All of the above
The differentiatial white cell count entails;
Enumerating the percentage of each white blood cell types
Enumerating the percentage of plasma
Enumerating the percentage of red blood cells
Reporting on the red cells only
None of the above
Increased intracellular levels of 2,3 DPG causes
Accident to the tissue
Shift to the right of the haemoglobin oxygen dissociation curve
Shift to left of the haemoglobin dissociation curve
increased in partial pressure of oxygen
All of the above
Decreased intracellular levels of 2,3 DPG causes
Shift to the bottom of the curve
Shift to the left of the curve
No shift at all.
Shift above
All of the above
Anti-AB is usually included in routine cell grouping for
Quality control
detection of subgroups of A
function at all
Detection of Haemolysis
Check forward grouping
Haemolysin can best be detected at
Room temperature
37°C
56°C
Forward grouping
Cold temperature
Window period refers to
Sweating in the night when the windows are closed
A time when the windows are opened and one catches cold in the night
A time between contacting an infection and the appearance of detectable antibodies
A time when a patient is tested positive for HIV
A time when antibodies begin to appear in serum
The recommended anticoagulant or preservatives for blood bank is
Acid citrate dextrose
citrate phosphate dextrose
Citrate phosphate dextrose adenine
Heparin
EDTA
What volume of CPDA is in a blood bag
63ml
450ml
250ml
0.5ml
100ml
A blood group O individual has what antigen?
No antigens
Antigen A and B
Antigen A only
Antigen B only
Antigen H only
What is the difference between DCT and ICT
DCT is used to detect red cell antibodies invivo
DCT is used to detect irregular antibodies in serum
ICT is used to detect red cells sensitized invivo
ICT is used to detect irregular antibodies in serum
No difference between DCT and ICT
In the HB genotype test, water is used to
wash the red blood cells
Preserve the red blood cells
Dilute the red cell suspension if too thick
Haemolyse the red blood cells
To release haemoglobin
Ammonium oxalate solution is used for counting of
white blood cells
Red blood cells
Reticulocytes
Sickle cells
Platelets
Too small an amount of anticoagulant to red cells can lead to
Clot formation
Haemolysis
Agglutination
Precipitation
Atypical reaction
Staining of Reticulocytes is known as
Supravital staining
Cytochemical staining
Differential staining
Preferential staining
Gram staining
Ketonuria is detected in urine by
Gilbert's test
Rotheras test
Sodium nitroprusside test
Glucose oxidase test
Benedict's test
The following anticoagulant can be used to collect blood sample for glucose estimation
Heparin
EDTA
Sodium/potassium oxidate
Flouride oxalate
Sodium citrate
The clinical chemistry laboratory is required to have
Safety showers
eyewash station
fires
Dressing room
Hazards
The biosafety level of a laboratory is based on the
Operations performed
Routes of transmission of infectious agents
Laboratory function or activity
Some of the above
None of the above
The part(s) of the body most frequently subject to injury in the clinical laboratory is/ are
Eyes
Skin
Mouth
Digestive tract
Respiratory tract
In chloride estimation, the following reagents are applicable
Silver nitrate
Ammonia
4-amino antipyrine
Potassium iodide
Acid bond
Consider the following about fire/ fire extinguishers
Halon for class C
Carbon (iv) oxide for class B
Carbon (iv) oxide for C
Pressurised water for class D
Dry chemicals for class A only
Consider the following about micropipette volume (micro litter) and colour
0.1-2.5/ blue
2.0-20/red
10-100/grey
50-200/ yellow
500-2500/red
About incubators
Calibration is necessary
Very operational temperature
use them in the presence of combustible materials
personal protective materials are needed
They work by heat transfer
About spectrophotometers, spectrum range of wavelength include
Ultraviolet is 10-200nm
Near ultraviolet is 0-100nm
Visible light is 38-780nm
Near infrared is 780-3000nm
Tar infrared is 30,000-300,000
Atomic absorption spectrophotometer is used to estimate the following
Bicarbonate
lead
magnesium
potassium
Chloride
In Glucose estimation in blood the following can be used to read the absorbance
Colorimeter
Spectrophotometer
Flourometer
Nephelometer
Lovibond
Flame photometer is used to estimate the following
Chloride
Lithium
Sodium
magnesium
Aluminum
The following are parameters used to evaluate the functions of the liver
Carbohydrates
Gamma glutamyl transferase
Alanine amino transferase
5' Nucleotidase
Acid phosphate
Glucosuria can occur in
A patient with glucose level greater than 10mmol/l
A patient with glucose immediately greater than 7.0mm/l
A patient with normal renal threshold
A patient with reduced renal threshold
A patient with raised renal threshold
The following are laboratory glassware
Eriemeyer flask
Griffin flask
Filtering flask
All of the above
None of the above
Common disiccants include
Magnesium perchlorate
Barium chloride
Alumina
phosphorus pentoxide
Calcium sulphate
About arterial blood samples
Blood gases can be measured
pH cannot be measured
Syringe are used instead of evacuated tubes
Partial pressure cannot be measured
None of the above
Analytical testing of blood involves the use of
Red blood cells
Whole blood
White blood cells
Plasma
Platelet
The following anticoagulants are used in clinical chemistry laboratory
Sodium Heparin
Lithium Heparin
Ammonium Heparin
Iodoacetate
All of the above is true
