WorksheetsMICRO-18
Total questions: 108
Worksheet time: 54mins
Name
Class
Date
1.
Purpose: Identifies Gram negative
a)
Gram sure
b)
Oxidase test
c)
Oxidation and fermentation
d)
Nitrate reduction test
2.
Purpose: Differentiates gram negative bacilli
a)
Gram sure
b)
Oxidase test
c)
Oxidation and fermentation
d)
Nitrate reduction test
3.
Purpose: Tests bacteria for their oxidative or fermentative ability
a)
Gram sure
b)
Oxidase test
c)
Oxidation and fermentation
d)
Nitrate reduction test
4.
Purpose: For all Enterobacterales members (all positive)
a)
Gram sure
b)
Oxidase test
c)
Oxidation and fermentation
d)
Nitrate reduction test
5.
Purpose: Further test for Enterobacterales members
a)
Nitrite reduction test
b)
Moeller's decarboxylase test
c)
Lysine Iron Agar (LIA)
d)
Triple Sugar Iron Agar (TSIA)
6.
Purpose: Differentiation of Enterobacterales members
a)
Nitrite reduction test
b)
Moeller's decarboxylase test
c)
Lysine Iron Agar (LIA)
d)
Triple Sugar Iron Agar (TSIA)
7.
Differentiation of Enterobacterales members through lysine
a)
Nitrite reduction test
b)
Moeller's decarboxylase test
c)
Lysine Iron Agar (LIA)
d)
Triple Sugar Iron Agar (TSIA)
8.
Purpose: Differentiation of Enterobacterales members through fermentation of sugars
a)
Nitrite reduction test
b)
Moeller's decarboxylase test
c)
Lysine Iron Agar (LIA)
d)
Triple Sugar Iron Agar (TSIA)
9.
Purpose: For gelatinase producers: Moraxella lacunata, Serratia orodifera, Proteus spp., Pseudomonas
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
10.
Positive result (QC organism): Partial (top layer) or total liquefaction of tube at 4oC (Bacillus subtilis)
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
11.
Other positive organisms: Salmonella arizonae/diarizonae, Shigella sonnei, Pantoea, Neisseria lactamica, Klebsiella, Citrobacter, Serratia, Y. enterocolitica, E. meningoseptica, E. coli, Enterobacter
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
12.
Other positive organisms: § Citrobacter § E. coli § PMP § Salmonella § Shigella
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
13.
Fermentation of any sugar in the slant in TSI would lead to?
a)
Yellow slant
b)
Purple slant
c)
Yellow butt
d)
Black precipitate
14.
PMP group
a)
K/K in LIA
b)
R/A or R/Y in LIA
15.
Klebsiella, E. coli, Edwardsiella, Salmonella (except Paratyphi), Serratia
a)
K/K in LIA
b)
R/A or R/Y in LIA
16.
Purpose: Distinguishes late lactose fermenter from non-lactose fermenters
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
17.
Purpose: IMViC test for differentiation of Enterobacterales
a)
Voges-Proskauer
b)
Citrate Utilization test
c)
Acetate / Malonate / Acetamide Utilization test
d)
Urease test
18.
Purpose: Presumptive ID of Proteus spp. and other enterobacteria
a)
Voges-Proskauer
b)
Citrate Utilization test
c)
Acetate / Malonate / Acetamide Utilization test
d)
Urease test
19.
Aka Christensen's method
a)
Voges-Proskauer
b)
Citrate Utilization test
c)
Acetate / Malonate / Acetamide Utilization test
d)
Urease test
20.
How long would you incubate in a closed container on MUG test at 35 to 37 degree Celsius?
a)
30 mins
b)
1 hr
c)
2 hrs
d)
3 hrs
21.
How long would you incubate in PAD test at 35 to 37 degree Celsius?
a)
12 to 24 hours
b)
18 to 24 hours
c)
21 to 48 hours
d)
48 to 72 hours
22.
Purpose: Detection of EHEC/E.coli O157:H7
a)
MUG test
b)
PAD or PDA test
c)
String test
23.
Indicator: 366 nm Ultraviolet light
a)
MUG test
b)
PAD or PDA test
c)
String test
24.
Medium: In glass slide + bile salt drop
a)
MUG test
b)
PAD or PDA test
c)
String test
25.
Purpose: Detects PMP/PPM group (all are positive)
a)
MUG test
b)
PAD or PDA test
c)
String test
26.
Purpose: Rapid detection of Vibrio spp.
a)
MUG test
b)
PAD or PDA test
c)
String test
27.
Purpose: IMViC test for differentiation of Enterobacterales
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
28.
Principle: Methylcoumarin + Gram neg’s aminopeptidase -> Blue fluorescence (Positive result)
a)
Gram Sure
b)
Oxidase test
c)
Oxidation and Fermentation
d)
Nitrate Reduction test
29.
Principle: Tetramethyl-p-phenylenediamine dihydrochloride + cytochrome oxidase à indophenol
a)
Gram Sure
b)
Oxidase test
c)
Oxidation and Fermentation
d)
Nitrate Reduction test
30.
Principle: 6 Carbohydrates: Glucose, Xylose, Mannitol, Maltose Lactose, Sucrose à Acids
a)
Gram Sure
b)
Oxidase test
c)
Oxidation and Fermentation
d)
Nitrate Reduction test
31.
Principle: Nitrate + bacterial nitrate reductase -> Nitrite
a)
Gram Sure
b)
Oxidase test
c)
Oxidation and Fermentation
d)
Nitrate Reduction test
32.
Principle: Nitrite to Nitrogen gas
a)
Nitrite Reduction test
b)
Moeller's decarboxylase test
c)
Lysine Iron Agar
d)
Triple Sugar Iron Agar
33.
Principle: Gelatin + gelatinase to liquefaction (refrigerated)
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
34.
Principle: ONPG (o-Nitrophenyl-β-D-Galactopyranoside) + β-galactosidase à o-nitrophenol (yellow)
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
35.
Principle: Tryptophan + tryptophanase indole (detected by indicators)
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
36.
Principle: Carbohydrate to mixed acids (pH <4.4)
a)
Gelatin hydrolysis
b)
ONPG test
c)
Indole test
d)
Methyl red test
37.
Principle: Carbohydrate to 2,3-Butanediol or acetoin (end product)
a)
Voges-Proskauer
b)
Citrate utilization test
c)
Acetate / Malonate / Acetamide Utilization test
d)
Urease test
38.
Principle: Tests ability to grow and convert Citrate to Ammonium PO4 and Ammonium hydroxide
a)
Voges-Proskauer
b)
Citrate utilization test
c)
Acetate / Malonate / Acetamide Utilization test
d)
Urease test
39.
Principle: Tests the ability of the bacteria to use the Sodium acetate or malonate or acetamide as sole source of carbon
a)
Voges-Proskauer
b)
Citrate utilization test
c)
Acetate / Malonate / Acetamide Utilization test
d)
Urease test
40.
Principle: Urea + urease to Ammonium carbonate (alkaline, pH >8.1)
a)
Voges-Proskauer
b)
Citrate utilization test
c)
Acetate / Malonate / Acetamide Utilization test
d)
Urease test
41.
Principle: 4-methylumbelliferyl-β-d-glucuronide + β-d-glucuronidase to 4-methylumbelliferyl
a)
MUG test
b)
PAD or PDA test
c)
String test
42.
Principle: Phenylalanine + Phenylalanine deaminase to ammonia and phenylpyruvic acid
a)
MUG test
b)
PAD or PDA test
c)
String test
43.
Principle: 0.5% Sodium deoxycholate lyses DNA of VIBRIO only, forming a string
a)
MUG test
b)
PAD or PDA test
c)
String test
44.
What is the positive result QC organism in Gram sure?
a)
Staphylococcus aureus
b)
Escherichia coli
c)
Pseudomonas aeruginosa
d)
Proteus mirabilis
45.
What is the positive result QC organism in Nitrate reduction test?
a)
Staphylococcus aureus
b)
Escherichia coli
c)
Pseudomonas aeruginosa
d)
Proteus mirabilis
46.
What is the positive result QC organism in Gelatin hydrolysis?
a)
Bacillus subtilis
b)
Shigella sonnei
c)
Escherichia coli
d)
Klebsiella aerogenes
47.
What is the positive result QC organism in ONPG test?
a)
Bacillus subtilis
b)
Shigella sonnei
c)
Escherichia coli
d)
Klebsiella aerogenes
48.
What is the positive result QC organism in Indole test?
a)
Bacillus subtilis
b)
Shigella sonnei
c)
Escherichia coli
d)
Klebsiella aerogenes
49.
What is the positive result QC organism in Methyl red test?
a)
Bacillus subtilis
b)
Shigella sonnei
c)
Escherichia coli
d)
Klebsiella aerogenes
50.
What is the positive result QC organism in Voges-Proskauer test?
a)
Bacillus subtilis
b)
Shigella sonnei
c)
Escherichia coli
d)
Klebsiella aerogenes
51.
What is the positive result QC organism in Citrate utilization test?
a)
Bacillus subtilis
b)
Shigella sonnei
c)
Escherichia coli
d)
Klebsiella aerogenes
52.
What is the positive result QC organism in string test?
a)
Proteus vulgaris
b)
All E. coli except E. coli O157
c)
Proteus mirabilis
d)
Vibrio spp.
53.
What is the positive result QC organism in PAD or PDA test?
a)
Proteus vulgaris
b)
All E. coli except E. coli O157
c)
Proteus mirabilis
d)
Vibrio spp.
54.
What is the positive result QC organism in MUG test?
a)
Proteus vulgaris
b)
All E. coli except E. coli O157
c)
Proteus mirabilis
d)
Vibrio spp.
55.
What is the positive result QC organism in Urease test?
a)
Proteus vulgaris
b)
All E. coli except E. coli O157
c)
Proteus mirabilis
d)
Vibrio spp.
56.
What is the positive result QC organism in Oxidase test?
a)
Staphylococcus aureus
b)
Escherichia coli
c)
Pseudomonas aeruginosa
d)
Proteus mirabilis
57.
What is the positive result QC organism in Nitrite reduction test?
a)
Staphylococcus aureus
b)
Escherichia coli
c)
Pseudomonas aeruginosa
d)
Proteus mirabilis
58.
Pseudomonas
a)
Oxidase positive
b)
Oxidase negative
59.
Aeromonas
a)
Oxidase positive
b)
Oxidase negative
60.
Enterobacterales
a)
Oxidase positive
b)
Oxidase negative
61.
Stenotrophomonas
a)
Oxidase positive
b)
Oxidase negative
62.
Acinetobacter
a)
Oxidase positive
b)
Oxidase negative
63.
Campylobacter
a)
Oxidase positive
b)
Oxidase negative
64.
Vibrio
a)
Oxidase positive
b)
Oxidase negative
65.
Pseudomonas aeruginosa
a)
Oxidizer
b)
Fermenter
66.
Escherichia coli
a)
Oxidizer
b)
Fermenter
67.
What is the indicator used in oxidation and fermentation test?
a)
Neutral red
b)
Bromothymol blue
c)
Malachite green
d)
Phenol red
68.
What is the indicator used in Urease test?
a)
Neutral red
b)
Bromothymol blue
c)
Malachite green
d)
Phenol red
69.
What is the indicator used in Acetate / Malonate / Acetamide Utilization test?
a)
Neutral red
b)
Bromothymol blue
c)
Malachite green
d)
Phenol red
70.
ɑ-naphthol
a)
Solution A (VP)
b)
Solution B (VP)
71.
KOH
a)
Solution A (VP)
b)
Solution B (VP)
72.
Medium used in indole test at 20 to 25 degree Celsius
a)
Agar deep + 1carbohydrate; Promote Anaerobiosis with Sterile melted petrolatum or paraffin oil
b)
Peptone broth
c)
Moeller’s decarboxylase medium, add 4mm Mineral oil
d)
SIM
e)
Tryptophan broth
73.
Medium used in indole test at 35 degree Celsius
a)
Agar deep + 1carbohydrate; Promote Anaerobiosis with Sterile melted petrolatum or paraffin oil
b)
Peptone broth
c)
Moeller’s decarboxylase medium, add 4mm Mineral oil
d)
SIM
e)
Tryptophan broth
74.
What is the medium in Moeller's decarboxylase test?
a)
Agar deep + 1carbohydrate; Promote Anaerobiosis with Sterile melted petrolatum or paraffin oil
b)
Peptone broth
c)
Moeller’s decarboxylase medium, add 4mm Mineral oil
d)
SIM
e)
Tryptophan broth
75.
What is the medium in Nitrite reduction test?
a)
Agar deep + 1carbohydrate; Promote Anaerobiosis with Sterile melted petrolatum or paraffin oil
b)
Peptone broth
c)
Moeller’s decarboxylase medium, add 4mm Mineral oil
d)
SIM
e)
Tryptophan broth
76.
What is the medium in Nitrate reduction test?
a)
Agar deep + 1carbohydrate; Promote Anaerobiosis with Sterile melted petrolatum or paraffin oil
b)
Peptone broth
c)
Moeller’s decarboxylase medium, add 4mm Mineral oil
d)
SIM
e)
Tryptophan broth
77.
What is the medium in Oxidation and fermentation test?
a)
Agar deep + 1carbohydrate; Promote Anaerobiosis with Sterile melted petrolatum or paraffin oil
b)
Peptone broth
c)
Moeller’s decarboxylase medium, add 4mm Mineral oil
d)
SIM
e)
Tryptophan broth
78.
Indicator: Durham tube – Detects N2 gas
a)
Nitrate reduction test
b)
Nitrite reduction test
79.
Indicator: Solution A: Sulfanilic acid and Solution B: alpha-naphthylamine; Durham tube – Detects N2 gas
a)
Nitrate reduction test
b)
Nitrite reduction test
80.
Checker: No color change after addition of indicator indicates a negative reaction, to further confirm, add zinc dust – it will convert nitrate into nitrite changing the color into red (true negative ___ reduction test)
a)
Nitrate reduction test
b)
Nitrite reduction test
81.
Colorless or gas production is a positive result in?
a)
Nitrate reduction test
b)
Nitrite reduction test
82.
Red color after the addition of indicator is a positive result in?
a)
Nitrate reduction test
b)
Nitrite reduction test
83.
Lysine
a)
Cadaverine
b)
Citrulline
c)
Putrescine
84.
Arginine
a)
Cadaverine
b)
Citrulline
c)
Putrescine
85.
Ornithine
a)
Cadaverine
b)
Citrulline
c)
Putrescine
86.
What is the indicator in Moeller's decarboxylase test?
a)
Bromothymol blue
b)
Neutral red
c)
Cresol red
d)
Bromocresol purple
87.
What is the positive result in Moeller's decarboxylase test?
a)
Red
b)
Yellow
c)
Purple
d)
Green
e)
Blue
88.
What is the positive result in ONPG test?
a)
Red
b)
Yellow
c)
Purple
d)
Green
e)
Blue
89.
What is the positive result in Methyl red test?
a)
Red
b)
Yellow
c)
Purple
d)
Green
e)
Blue
90.
What is the positive result in Voges-Proskauer test?
a)
Red
b)
Yellow
c)
Purple
d)
Green
e)
Blue
91.
What is the positive result in Acetate / Malonate / Acetamide Utilization test?
a)
Red
b)
Yellow
c)
Purple
d)
Green
e)
Blue
92.
What is the positive result in PAD or PDA test?
a)
Red
b)
Yellow
c)
Purple
d)
Green
e)
Blue
93.
What is the positive result in Urease test?
a)
Red
b)
Yellow
c)
Purple
d)
Green
e)
Blue
94.
What is the positive result in indole test?
a)
Pink ring or red ring
b)
Blue ring or purple ring
c)
Black ring or brown ring
d)
Yellow ring or orange ring
95.
In gelatin hydrolysis, gelatin media serves as?
a)
Medium
b)
Indicator
c)
Either
d)
Neither
96.
Oxidation and Fermentation may be incubated at which temperature and for how long?
a)
22 to 25 degree Celsius for up to 3 days
b)
22 to 25 degree Celsius for up to 7 days
c)
35 to 37 degree Celsius for up to 3 days
d)
35 to 37 degree Celsius for up to 7 days
97.
Urease test involve incubation at which temperature and for how long?
a)
22 to 25 degree Celsius for up to 3 days
b)
22 to 25 degree Celsius for up to 7 days
c)
35 to 37 degree Celsius for up to 3 days
d)
35 to 37 degree Celsius for up to 7 days
98.
Acetate / Malonate / Acetamide Utilization test involve incubation at which temperature and for how long?
a)
22 to 25 degree Celsius for up to 3 days
b)
22 to 25 degree Celsius for up to 7 days
c)
35 to 37 degree Celsius for up to 3 days
d)
35 to 37 degree Celsius for up to 7 days
99.
How many drops of BHI culture is used on urease test?
a)
1
b)
2
c)
3
d)
4
100.
What is the concentration of Sodium deoxycholate used in string test?
a)
0.5% sodium deoxycholate
b)
1.5% sodium deoxycholate
c)
2.5% sodium deoxycholate
d)
10.5% sodium deoxycholate
101.
Indicator in PAD test
a)
2% FeCl3
b)
5% FeCl3
c)
10% FeCl3
d)
15% FeCl3
102.
For Enterobacterales
a)
Kovac's reagent
b)
Ehrlich's reagent
103.
Composition: dimethylamine-benzaldehyde & HCl
a)
Kovac's reagent
b)
Ehrlich's reagent
104.
For other Gram neg bacilli and Anaerobes methylamine-benzaldehyde & HCl
a)
Kovac's reagent
b)
Ehrlich's reagent
105.
Composition: dimethylamine-benzaldehyde & HCl, Absolute ethanol, xylene
a)
Kovac's reagent
b)
Ehrlich's reagent
106.
How long is the incubation time in indole test at 35 to 37 degree Celsius?
a)
24 hours
b)
48 hours
c)
72 hours
d)
Any of the choices
107.
How long is the incubation time in Methyl red test at 35 to 37 degree Celsius?
a)
24 hours
b)
48 hours
c)
72 hours
d)
Any of the choices
108.
Incubation time in ONPG test at 37 degree Celsius
a)
3 to 4 hours
b)
4 to 6 hours
c)
6 to 8 hours
d)
5 to 6 hours
100 %
