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WorksheetsBiol 1307 DC Interactive
Total questions: 15
Worksheet time: 8mins
This figure shows the recognition sequence for EcoRI. Which of the following statements regarding recombinant DNA is false?
Restriction enzymes can create DNA fragments with “sticky ends.”
A single restriction enzyme can cut DNA sequences at several different recognition sites.
Restriction enzymes cut DNA at palindromic sequences.
Restriction enzymes can create DNA fragments with “blunt ends.”
Complementary sticky ends can form hydrogen bonds with one another.
In this figure showing a bacterial defense system against viral attack, label 1 points to _______, and label 2 points to _______.
ligase; methylase
ligase; a methyl group
methylase; a methyl group
a restriction enzyme; methylase
a restriction enzyme; a methyl group
. Suppose a piece of DNA is digested with two different restriction enzymes in three reactions. Reactions 1 and 2 contain only a single enzyme, and the third reaction receives both enzymes. The reactions are loaded into wells of an electrophoresis gel. Wells 1 and 2 receive the reactions with single enzymes, whereas well 3 gets the reaction with both enzymes. The figure here shows the results of the electrophoresis run. Which of the following choices correctly describes the relationship between the fragment size of DNA in the bands (A–E)?
C = A + B
B = D + A
B = D + E
C = A + D
A + B = D
Which of the following statements about DNA ligase is false?
DNA ligase plays an important role in DNA replication.
DNA ligase forms covalent bonds between DNA fragments.
DNA ligase is an essential tool in recombinant DNA technology.
DNA ligase is found only in prokaryotes.
DNA ligase can join together DNA fragments from different organisms.
Which of the following is not an important characteristic of a bacterial plasmid vector (shown)?
It should have the ability to integrate into the host’s genome.
It should have a reporter gene.
It should have a gene for a selectable marker.
It should be small in size.
It should have the ability to amplify the gene being carried by producing many copies within the host cell.
Which of the following statements about the Ti plasmid is false?
It is present in the bacterium, Agrobacterium tumefaciens.
It inserts some its genes into an infected plant cell genome.
It has genes that induce tumor formation in plants.
It is by itself an infectious agent to plants.
The tumor-inducing genes are removed before using the plasmid as a vector.
Which of the following statements about reporter genes is false?
Reporter genes are a kind of selectable marker.
Reporter genes can be used to study promoter function.
The gene for green fluorescent protein (GFP) may be attached to genes for other proteins.
The gfp gene does not have to be expressed to serve as a reporter.
GFP can be used to study the targeting of other protein molecules to compartments within eukaryotic cells.
Select the statement that has the most significance for the experiment shown.
E coli can be either sensitive or resistant to specific antibiotics.
Antibiotic resistance may be conferred to the bacterium by genes in plasmids.
The foreign DNA does not transform the bacterium.
Transformed bacteria that contain foreign DNA may be identified by insertion of the foreign DNA into an antibiotic resistance gene.
Antibiotic resistance genes are good selectable markers.
A cDNA library differs from a genomic DNA library in that
reverse transcriptase is needed to make a cDNA library.
a cDNA library from a eukaryote is smaller than a genomic library from the same organism.
a cDNA library contains only genes that are transcribed.
a cDNA library is made from mRNA.
All of the above
Which of the following statements about gene inactivation by homologous recombination (HR) is false?
HR is used as a “shotgun” approach to mutagenesis.
HR can be used to inactivate genes.
HR is a type of insertional mutagenesis.
HR is used to make “knockout mice.”
HR provides a targeted approach to mutagenesis.
This figure shows the inhibition of mRNA transcription using antisense technology. The red molecule in this example is called _______, and it inhibits translation by _______.
small interference RNA; stimulating the breakdown of mRNA
small interference RNA; forming a stable complex with the mRNA target
antisense RNA; stimulating the breakdown of mRNA
antisense RNA; forming a stable complex with the mRNA target
reverse transcriptase; stimulating the breakdown of mRNA
Which of the following is not a valid statement regarding DNA microarray technology (shown here)?
DNA microarray technology allows the determination of mRNA expression patterns.
DNA microarrays contain a single type of DNA, allowing for the hybridization of only a single gene at a time.
When cellular mRNA is analyzed, cDNA must first be made prior to hybridization with the DNA microarray.
The cDNA molecules used in an experiment must first be labeled with a fluorescent marker (dye) prior to hybridization with the DNA microarray.
DNA microarrays may be used for detecting genetic variants.
Which of the following features is normally not required in an expression vector designed to be inserted into E. coli?
Bacterial promoter for RNA polymerase binding
Terminator for transcription
Poly A–addition sequence
Ribosome-binding site
ori
Which of the following statements about the application of biotechnology used to produce human insulin, is false?
Recombinant DNA technology allowed for insulin production in large enough quantities for commercial manufacture.
The insulin expression vectors had to be designed for use in a prokaryotic cell.
The insulin produced by biotechnology differs from that made naturally.
Insulin is present in small amounts in humans.
Antibiotic resistance was used as a selectable marker.
Which of the following statements about the advantages of the application of biotechnology to the improvement of crop plants over traditional breeding methods is false?
Recombinant DNA technology allows the identification of specific genes.
Recombinant DNA technology allows for the introduction of any gene from any organism into the crop plant.
Recombinant DNA technology is especially effective in altering traits controlled by multiple genes.
New plants may be generated quickly by recombinant DNA technology.
Traditional breeding is a “hit-or-miss affair.”
