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Spring 2026 Benchmark Review

Total questions: 50

Worksheet time: 1hrs 5mins

Name
Class
Date
1.

In cellular respiration, ​ (a)   is broken down, ​ (b)   energy.

Choose from the below words
glucose
releasing
carbon dioxide
storing
creating
oxygen
water
2.

Catergorize the following molecules for the process that transforms solar energy into chemical energy.

Categorize the following

carbon dioxide

water

sunlight

oxygen

glucose

Reactants
Products
3.

Catergorize the following molecules for the process that transforms chmical energy into cellular energy.

Categorize the following

carbon dioxide

water

ATP

oxygen

glucose

Products
Reactants
4.

Choose the correct part of the Cell Cycle where DNA replication takes place.

5.

Choose the correct part of the Cell Cycle where the cell is growing and preparing for division.

6.

Order the steps of protein synthesis from beginning to end.

a)

DNA unzips

b)

TRANSCRIPTION

c)

mRNA leaves the nucleus

d)

TRANSLATION

e)

protein is folded and modified

1)
2)
3)
4)
5)
7.

Match each biomolecule to its purpose

a)

store and release energy, structural support of plants

1.

carbohydrates

b)

builds cell membranes, waterproof coverings, insulation

2.

lipids

c)

store genetic information

3.

nucleic acids

d)

cell transport, fight diseases, change rate of chemical reactions

4.

proteins

8.

Choose all TRUE statements.

a)

Transcription occurs in the nucleus.

b)

Translation occurs in the cytoplasm.

c)

Transcription occurs on the ribosome.

d)

Translation occurs in the endoplasmic reticulum.

9.

Choose all TRUE statements.

a)

DNA and all types of RNA are built of repeating monomers called nucleotides.

b)

DNA and all types of RNA are built of repeating monomers called nucleic acids.

c)

In DNA, adenine always pairs with uracil.

d)

In DNA, adenine always pairs with thyamine.

10.

What is the purpose of DNA replication?

a)

To ensure genetically identical cells

b)

To create more proteins

c)

To use up excess ATP

d)

To build subunits called nucleotides

11.

Match each process to its correct description.

a)

DNA makes an exact copy of itself

1.

replication

b)

DNA used to make a template of mRNA

2.

transcription

c)

mRNA used to determine order of amino acids

3.

translation

12.

What gas is produced during photosynthesis?

a)

oxygen

b)

carbon dioxide

c)

glucose

d)

water

13.

Amino Acids are the

a)

building blocks of proteins

b)

monomers of nucleic acids

c)

waterproof covering on cell membranes

d)

enzymes that speed up chemical reactions

14.

Categorize the different types of cellular respiration.

Categorize the following

produces more energy

less efficient

requires oxgyen

does not require oxygen

produces ATP

begins with glucose

Aerobic Respiration
Anaerobic Respiration
BOTH
15.

Choose the stage of mitosis where chromosomes are aligned at the equator of the cell.

16.

What process is shown in this diagram?

a)

mitosis

b)

cell differentiation

c)

meiosis

d)

genetic mutations

17.

What products of the light-dependent reaction of photosynthesis are used in the Calvin Cycle?

a)

ATP

b)

ADP

c)

NADPH

d)

NADP+

e)

CO2

18.

Which of the following is produced in the light-dependent reaction but not used in the Calvin Cycle?

a)

ATP

b)

NADPH

c)

H2O

d)

O2

19.

When a person eats eggs, meat, or other sources of protein, how does their body use the protein to carry out protein synthesis?

a)

The proteins are broken down into amino acids, then reassembled according to the person's DNA.

b)

The proteins are broken down into amino acids and then absobed into pre-existing proteins.

c)

The proteins are the same for all organisms, and just get reused.

d)

The proteins are refolded into human proteins.

e)

The proteins release amino acids and then the amino acids are changed into human amino acids.

20.

Can matter be created or destroyed?

a)

Yes

b)

No

c)

It depends

d)

Sometimes

21.

If I set up the terrarium pictured and measured the mass to be 1065 grams. Sunlight can enter the system, but nothing else can enter or exit the glass.

After 2 weeks, I measure the mass again. What should it be and why?

a)

Equal to 1065 grams because no outside matter entered the system.

b)

More than 1065 grams because plants did photosynthesis, creating glucose, which has more mass than carbon dioxide.

c)

Less than 1065 grams because plants did photosynthesis, releasing carbon dioxide, which has no mass.

22.
What amino acid is represented by the codon UUA?
a)
Phenylalanine
b)
Tyrosine
c)
Leucine
d)
Stop codon
23.

What was the original DNA that matched with this RNA sequence: AGU ACA ?

a)

UCA UGU

b)

AGT ACA

c)

TCA TGT

d)

AGU ACA

24.

Complete the process of Transcription by using the following DNA sequence...

ATG CAG CCA

a)

UAC GUC GGU

b)

UTC GUC GGU

c)

TAC GGG GGU

d)

TAT GUC GGU

25.

Using the codon chart, please indicate the amino acid sequence that will be produced from this mRNA sequence: AUG UUC GAU

a)

GLU - PHE - ASP

b)

MET - ARG - PHE

c)

MET - PRO - ASP

d)

MET - PHE - ASP

26.

Do humans completely digest food while chewing?

a)

Yes

b)

No

27.

Cellular Respiration converts nutrients from food into usable energy.

a)

True

b)

False

28.

Human muscles produce glucose during exercise.

a)

True

b)

False

29.

Match the biomolecules to their functions

a)

store energy and form protective barriers in cell membranes

1.

lipids

b)

provide immediate energy to the body

2.

carbohydrates

c)

carry out cellular functions, speed up chemical reactions, build living things

3.

proteins

d)

code for proteins

4.

nucleic acids

30.

Select the part of the image below that is incorrect.

31.

Label the components of the diagram.

32.

Choose the arrow that shows photosynthesis.

33.

Choose the arrow that shows respiration.

34.

The image shows an experiment where students are measuring dissolved oxygen and carbon dioxide levels in different flasks. 4 flasks were placed in light and 4 were placedin the dark.

They placed the following in each flask:

A and E: 2 snails

B and F: 2 plants

C and G: 2 snails and 2 plants

D and H: water only

Which flask should have the most dissolved oxygen after 1 week?

a)

A

b)

B

c)

F

d)

C

e)

G

35.

The image shows an experiment where students are measuring dissolved oxygen and carbon dioxide levels in different flasks. 4 flasks were placed in light and 4 were placedin the dark.

They placed the following in each flask:

A and E: 2 snails

B and F: 2 plants

C and G: 2 snails and 2 plants

D and H: water only

Which flask should have the most carbon dioxide after 1 week?

a)

A

b)

B

c)

F

d)

C

e)

G

36.

Organize these options into the right categories

Categorize the following

passed from parent to offspring

stores genetic information

speeds up chemical reactions

insulin, hemoglobin, antibodies

builds skin, hair and nails

provides insulation and waterproofing

builds cell membranes

stores energy

main source of energy for cells

builds cell walls

Carbohydrate
Lipid
Protein
Nucleic Acid
37.

What type of organism is represented by the protein synthesis model in the image?

a)

animal

b)

plant

c)

fungi

d)

we can't tell because all organisms have the same 4 nucleotides in DNA and use transcription and translation to build proteins

38.

The CENTRAL DOGMA of biology states that genetic information flows only in one direction, from DNA, to RNA, to protein.

Select the statement that is supported by this theory.

a)

DNA is found in the cells of all organisms.

b)

DNA contains instructions for building proteins.

c)

The beginning and end of polypeptide chains are determined by specific DNA sequences.

d)

tRNA and rRNA are essential to the process of transcription.

39.

Which of the following is are true statements? SELECT 2

a)

Anaerobic respration does not require oxygen and takes place in the cell's cytoplasm.

b)

Anaerobic respration does not require oxygen and takes place in the cell's mitochondria.

c)

Aerobic respration does require oxygen and takes place in the cell's cytoplasm.

d)

Aerobic respration does require oxygen and takes place in the cell's mitochondria.

40.

Organize these options into the right categories

Categorize the following

starch

lipids

cellulose

fats, oils, waxes

enzymes

mRNA

ribosomes

DNA

Can be synthesized from only glucose
Need the addition of other molecules
41.

Match each image to the correct steps happening in mitosis.

a)

1.

chromosomes are moving toward the poles

b)

2.

nuclear membrane is breaking down

c)

3.

chromosomes are lined up at the equator

d)

4.

nuclear membrane has started to reappear

42.
  1. Complete the following explaination of protein synthesis.

  2. ​ (a)   contains all the instructions an organism needs to live.  During ​ (b)   , DNA is used as a template to make a molecule of messenger RNA (mRNA) which then leaves the ​ (c)   and goes to a ribosome in the cytoplasm. During ​ (d)   , the genetic code in mRNA is read and used to put ​ (e)   in place to make a protein.

Choose from the below words
translation
DNA
transcription
nucleus
amino acids
43.

How is energy stored before being used in respiration?

a)

chemical energy

b)

kinetic energy

c)

cellular energy

d)

heat energy

44.

How is cell differentiation involved in this image?

a)

When cells develop, they remove parts of their genetic material to become specialized cells.

b)

All cells in an organism have the same genetic information, but only activate specific genes depending on the type of cell it has become.

c)

Stem cells have transferred energy into the more complex cells to build more genetic material and become more advanced.

d)

Fully developed cells have collected cellular parts from other cells to become specialized.

45.

Determine if the following are true or false for the monomers pictured.

Categorize the following

All contain oxygen

All contain hydrogen

All contain a ring-shaped section

All contain nitrogen

All contain equal amounts of oxygen atoms

True
False
46.

Why does the cell cycle have checkpoints?

a)

To prevent uncontrolled cell division.

b)

To regulate a body's ability to metabolize sugars.

c)

To detect damage in DNA.

d)

To prevent the cell cycle from happening out of order.

e)

To provide energy and support during the cell cycle.

47.

_ is the type of energy used by cells.

(a)  

48.

Match the following:

a)

C6H12O6 + O2

1.

products of photosynthesis

b)

H2O + CO2

2.

products of cellular respiration

c)

chloroplast

3.

site of photosynthesis

d)

mitochondria

4.

site of cellular respiration

49.

Match the following:

a)

C6H12O6 + O2

1.

reactants of cellular respiration

b)

H2O + CO2

2.

reactants of photosynthesis

c)

chloroplast

3.

site of photosynthesis

d)

mitochondria

4.

site of cellular respiration

50.

Sickle cell anemia is caused by one base being changed from T to A.

This causes the amino acid to change from GLU to VAL.

How does this impact the final protein produced?

a)

The change in amino acid results in a differently shaped protein, which impacts the protein's function.

b)

The change in amino acid causes the protein to stop synthesizing too early, resulting in a shorter protein.

c)

The change in DNA removes blood cells from the body, which prevents oxygen from reaching the cells.

d)

The change in DNA stops the body from repairing bone marrow, which prevents the production of healthy blood cells.