WorksheetsRecombinant DNA technology
Total questions: 20
Worksheet time: 20mins
What is a recombinant DNA?
DNA from 2 different organisms combined
DNA from 2 different ribosomes
DNA that is separated out from a vector
Which of the following could be used as a vector for a human gene?
Bacterial Plasmid
Bacteria
A mitochondria
mRNA
A circular piece of DNA, found in bacteria, is called:
A plasmid
mRNA
Ribosome
Nucleotide
Bacteria cells can be used to produce insulin for the treatment of diabetes in humans. Which best explains how the bacteria cells are able to produce insulin for humans?
The bacteria have been grown on a petri dish containing insulin.
The bacteria have been genetically engineered to contain the DNA needed to produce insulin.
The bacteria have been exposed to radioactivity to create mutant strains capable of producing insulin.
The bacteria have been grown on a petri dish containing sugar; the bacteria produce insulin in response to the sugar.
Which of the following is a protein that acts as DNA "scissors"?
plasmid
restriction enzyme
ligase
protein expression
Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?
amplification
insertion
transformation
screening
Restriction enzymes found in bacterial cells are naturally used:
During DNA replication.
To degrade the bacterial cell's DNA.
To degrade viral DNA that enters the cell.
To attach pieces of DNA together.
What is the purpose of amplification?
To screen for recombinant bacteria.
To join the rDNA with the host cell.
To produce multiple copies of the desired gene.
To prevent the bacteria from reproducing.
Restriction endonucleases cut DNA at a specific site called
ligation site
ori
recognition sequence
replication site
In genetic engineering, DNA ligase is used as:
a probe
a sealing enzyme
a restriction enzyme
a mutagen
An organism that has had its genes manipulated by genetic engineering is considered as ___.
transgenic
crossbred
transmutant
culitvars
Which of the following is used as selectable marker?
Ampicillin resistance gene
Plasmid resistance gene
Salmonella resistance gene
Penicillin resistance gene
What is the significance of the lacZ gene in cloning?
It provides antibiotic resistance
It is involved in DNA replication
It allows for blue-white screening
It encodes a fluorescent protein
What is the main function of PCR in recombinant DNA technology?
To ligate DNA
To sequence DNA
To isolate DNA
To amplify DNA
bacteriophages (bacterial viruses) participate in this gene transfer
transformation
conjugation
transduction
translation
