Bacterial Transformation

Front

What is genetic transformation?

Back

A host organism taking in foreign DNA

Front

What is the purpose of the Luria broth (LB) when performing genetic transformation?

Back

Provides nutrients needed for bacteria growth

Front

Which is not a characteristic of prokaryotic cells? Options: contains a nucleus, contains plasmid DNA, single-celled, enclosed by a cell wall

Back

contains a nucleus

Front

What is a "-amp^R" cell?

Back

A "-amp^R" cell does not have the amp^R gene in its plasmid and is not resistant to ampicillin.

Front

What does it mean for cells to be competent?

Back

Their cell walls are altered to allow DNA to pass more easily.

Front

What process increases the competency of cells? Options: calcium chloride and an ice bath, calcium chloride and heat shock, Luria broth (LB) and an ice bath, Luria broth (LB) and heat shock

Back

calcium chloride and heat shock

Front

Correct sequence for the process of genetic transformation?

Back

bacterial cells transferred to cold CaCl₂ , plasma with foreign DNA added bacteria , cells heat shocked at 42^oC , cells spread on agar petri dish , bacteria given time to grow on petri dish

Front

Which one of these agar plates contains bacteria most like the original bacterial culture? Options: +pGLO LB/amp, +pGLO LB/amp/ara, -pGLO LB/amp, -pGLO LB

Back

-pGLO LB

Front

Which one of these agar plates would there be no bacteria growth? +pGLO LB/amp, +pGLO LB/amp/ara, -pGLO LB/amp, -pGLO LB

Back

-pGLO LB/amp

Front

Which image is a plasmid?

Back