MBT FL24 Transformation
Flashcard
•
Science
•
12th Grade
•
Practice Problem
•
Hard
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15 questions
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1.
FLASHCARD QUESTION
Front
What is transformation in genetic engineering?
Back
The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.
2.
FLASHCARD QUESTION
Front
Why is calcium chloride used in the transformation process?
Back
The cations from CaCl2 allow for the negative plasmid to get close to the negative plasma membrane.
3.
FLASHCARD QUESTION
Front
What is the correct sequence for the process of genetic transformation?
Back
1. Bacterial cells transferred to cold CaCl2 2. Plasmid with foreign DNA added to bacteria 3. Cells heat shocked at 42°C 4. Cells spread on agar petri dish 5. Bacteria given time to grow on petri dish.
4.
FLASHCARD QUESTION
Front
How can a human gene be expressed in a bacterial cell?
Back
The DNA structure and code is the same in all living organisms; therefore, bacteria contain the same cell machinery for performing transcription and translation and can read the same DNA as human cells.
5.
FLASHCARD QUESTION
Front
What process increases the competency of cells?
Back
Calcium chloride and heat shock.
6.
FLASHCARD QUESTION
Front
What is a plasmid?
Back
A plasmid is a small, circular piece of DNA that is separate from chromosomal DNA and can replicate independently within a bacterial cell.
7.
FLASHCARD QUESTION
Front
What role does heat shock play in transformation?
Back
Heat shock creates a temperature difference that helps to facilitate the uptake of plasmid DNA by bacterial cells.
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