Gel Electrophoresis Practice

Front

What is Gel Electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

Front

What is the principle behind Gel Electrophoresis?

Back

Molecules are subjected to an electric field, causing them to migrate through a gel matrix; smaller molecules move faster than larger ones.

Front

What is the role of the gel in Gel Electrophoresis?

Back

The gel acts as a molecular sieve, allowing smaller molecules to pass through more easily than larger ones.

Front

What types of samples can be analyzed using Gel Electrophoresis?

Back

DNA, RNA, and proteins.

Front

What is the purpose of a loading dye in Gel Electrophoresis?

Back

To visualize the sample and track its migration through the gel.

Front

What is the significance of the buffer solution in Gel Electrophoresis?

Back

It maintains the pH and ionic strength, allowing for proper conduction of electricity.

Front

What is the difference between agarose gel and polyacrylamide gel?

Back

Agarose gel is used for larger DNA fragments, while polyacrylamide gel is used for smaller fragments and proteins.

Front

What is a DNA ladder in Gel Electrophoresis?

Back

A set of known DNA fragments used as a reference to determine the size of unknown samples.

Front

How is the gel prepared for Gel Electrophoresis?

Back

Agarose or polyacrylamide is mixed with buffer, heated to dissolve, poured into a mold, and allowed to solidify.

Front

What is the role of the electric current in Gel Electrophoresis?

Back

It drives the migration of charged molecules through the gel.