Quick 10Q: BioTechnology

Front

What is the result of 1 DNA fragment after 5 cycles of PCR?

Back

32 fragments.

Front

What is the minimum number of restriction sites on a plasmid with 3 fragments on a gel?

Back

3 restriction sites.

Front

Why must primers be added to the thermocycler during PCR?

Back

Polymerase requires an open 3' end.

Front

Why are some bands darker in gel electrophoresis?

Back

There are a large amount of fragments the same size.

Front

What process allows for the amplification of a DNA fragment abiotically?

Back

Polymerase Chain Reaction (PCR).

Front

Define Polymerase Chain Reaction (PCR).

Back

A technique used to amplify DNA segments.

Front

What role do primers play in PCR?

Back

Primers provide a starting point for DNA synthesis.

Front

What is gel electrophoresis used for?

Back

To separate DNA fragments based on size.

Front

How does the number of cycles in PCR affect the amount of DNA produced?

Back

Each cycle doubles the amount of DNA, leading to exponential growth.

Front

What is a plasmid?

Back

A small, circular piece of DNA commonly used in genetic engineering.