Gel Electrophoresis Practice

Front

What is gel electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

Front

What is the role of the gel in gel electrophoresis?

Back

The gel acts as a medium through which molecules can move; smaller molecules move faster than larger ones.

Front

What is the purpose of the buffer solution in gel electrophoresis?

Back

The buffer maintains a stable pH and provides ions that carry the electric current.

Front

What is the significance of the DNA ladder in gel electrophoresis?

Back

The DNA ladder serves as a molecular weight marker to estimate the size of the DNA fragments.

Front

What is the charge of DNA molecules?

Back

DNA molecules are negatively charged due to their phosphate backbone.

Front

How does the size of DNA fragments affect their movement in gel electrophoresis?

Back

Smaller DNA fragments move faster and travel further through the gel than larger fragments.

Front

What is the purpose of staining in gel electrophoresis?

Back

Staining allows visualization of the DNA bands after electrophoresis.

Front

What is agarose gel?

Back

Agarose gel is a polysaccharide used to create the gel matrix for electrophoresis.

Front

What is the difference between agarose gel electrophoresis and polyacrylamide gel electrophoresis?

Back

Agarose gel is used for larger DNA fragments, while polyacrylamide gel is used for smaller fragments and proteins.

Front

What is the role of the power supply in gel electrophoresis?

Back

The power supply provides the electric current that drives the movement of charged molecules through the gel.