MBT ELISA & Antibodies

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What are Monoclonal antibodies?

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An antibody produced by a single clone of cells or cell line and consisting of identical antibody molecules.

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What is a primary antibody?

Back

Patient's antibody and initial step in ELISA.

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What is an antigen?

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A molecule on a pathogen to which an antibody can bind.

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What does the secondary antibody recognize in an ELISA test?

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The antigen-antibody complex.

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What cannot be determined by an ELISA test?

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Genetic sequence of a pathogen.

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What is the purpose of ELISA?

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To detect and quantify proteins, antibodies, or hormones in a sample.

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What is the role of the enzyme in an ELISA test?

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To produce a measurable signal, usually a color change, indicating the presence of the target molecule.

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What is the difference between direct and indirect ELISA?

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Direct ELISA uses a labeled primary antibody, while indirect ELISA uses a labeled secondary antibody.

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What is a capture antibody?

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An antibody that is immobilized on a solid surface to capture the target antigen from a sample.

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What is the significance of the standard curve in ELISA?

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It is used to determine the concentration of the target molecule in unknown samples.