Front

What are Monoclonal antibodies?

Back

An antibody produced by a single clone of cells or cell line and consisting of identical antibody molecules.

Front

What is a primary antibody?

Back

Patient's antibody and initial step in ELISA.

Front

What is an antigen?

Back

A molecule on a pathogen to which an antibody can bind.

Front

What does the secondary antibody recognize in an ELISA test?

Back

The antigen-antibody complex.

Front

What cannot be determined by an ELISA test?

Back

Genetic sequence of a pathogen.

Front

What is the purpose of ELISA?

Back

To detect and quantify proteins, antibodies, or hormones in a sample.

Front

What is the role of the enzyme in an ELISA test?

Back

To produce a measurable signal, usually a color change, indicating the presence of the target molecule.

Front

What is the difference between direct and indirect ELISA?

Back

Direct ELISA uses a labeled primary antibody, while indirect ELISA uses a labeled secondary antibody.

Front

What is a capture antibody?

Back

An antibody that is immobilized on a solid surface to capture the target antigen from a sample.

Front

What is the significance of the standard curve in ELISA?

Back

It is used to determine the concentration of the target molecule in unknown samples.