NC EOC Review: DNA Fingerprinting & Gel Electrophoresis

Front

What is the role of restriction enzymes?

Back

To cut the DNA into fragments.

Front

What are the pieces of DNA that are seen in the DNA fingerprint called?

Back

Fragments.

Front

In a DNA fingerprint, where can lighter-weight fragments be found?

Back

At the bottom of the gel.

Front

Which suspect matches that of the crime scene DNA?

Back

The suspect whose DNA profile matches the fragments from the crime scene.

Front

How does gel electrophoresis separate DNA fragments?

Back

By size, with smaller fragments moving faster and farther than larger ones.

Front

What is DNA fingerprinting used for?

Back

To identify individuals based on their unique DNA profiles.

Front

What is the purpose of using a DNA ladder in gel electrophoresis?

Back

To provide a reference for estimating the size of DNA fragments.

Front

What is the significance of the bands in a DNA fingerprint?

Back

They represent the different DNA fragments that can be compared between samples.

Front

What is the process of amplifying DNA before fingerprinting called?

Back

Polymerase Chain Reaction (PCR).

Front

What is the function of a loading dye in gel electrophoresis?

Back

To help visualize the DNA samples and track their movement through the gel.