CRISPR Lab Review
Flashcard
•
Science, Biology
•
11th - 12th Grade
•
Practice Problem
•
Hard
Wayground Content
FREE Resource
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15 questions
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1.
FLASHCARD QUESTION
Front
What is the function of sgRNA in CRISPR technology?
Back
sgRNA tells Cas9 where to cut in the DNA sequence.
2.
FLASHCARD QUESTION
Front
What is the main difference between pLZDonor and pLZDonorGuide plasmids?
Back
Only pLZDonorGuide has sgRNA.
3.
FLASHCARD QUESTION
Front
What happens to bacteria with NO arabinose, Cas9, and pLZDonorGuide?
Back
The bacteria would die.
4.
FLASHCARD QUESTION
Front
What occurs when the lacZ gene is functioning normally?
Back
B-gal breaks down X-gal.
5.
FLASHCARD QUESTION
Front
What color do bacteria appear when the lacZ gene is working normally?
Back
The bacteria appears blue.
6.
FLASHCARD QUESTION
Front
What is the role of Cas9 in CRISPR?
Back
Cas9 is an enzyme that cuts DNA at a specific location.
7.
FLASHCARD QUESTION
Front
What is the purpose of a Donor DNA Template in CRISPR?
Back
It provides the genetic material needed for repair after Cas9 cuts the DNA.
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