CRISPR Lab Review

Front

What is the function of sgRNA in CRISPR technology?

Back

sgRNA tells Cas9 where to cut in the DNA sequence.

Front

What is the main difference between pLZDonor and pLZDonorGuide plasmids?

Back

Only pLZDonorGuide has sgRNA.

Front

What happens to bacteria with NO arabinose, Cas9, and pLZDonorGuide?

Back

The bacteria would die.

Front

What occurs when the lacZ gene is functioning normally?

Back

B-gal breaks down X-gal.

Front

What color do bacteria appear when the lacZ gene is working normally?

Back

The bacteria appears blue.

Front

What is the role of Cas9 in CRISPR?

Back

Cas9 is an enzyme that cuts DNA at a specific location.

Front

What is the purpose of a Donor DNA Template in CRISPR?

Back

It provides the genetic material needed for repair after Cas9 cuts the DNA.

Front

What is X-gal used for in CRISPR experiments?

Back

X-gal is a substrate that is broken down by B-gal, producing a blue color.

Front

What happens to the color of bacteria when the lacZ gene is disrupted?

Back

The bacteria appears white.

Front

What is the significance of arabinose in CRISPR experiments?

Back

Arabinose is often used to induce the expression of genes, including those involved in CRISPR.