MI4.1.2 Safety & Mod 1 Pre-Lab

Front

What is the purpose of Module 1 in the lab?

Back

To transform the E. coli bacteria with the pGFP plasmid.

Front

What should students who are allergic to antibiotics do before participating in the lab?

Back

Speak with their teacher before the first day of the lab.

Front

How should inoculated plates be stored in the incubator overnight?

Back

Agar side up.

Front

Which tube should pGFP DNA be added to?

Back

+DNA tube.

Front

What personal protective equipment must be worn during the laboratory exercise?

Back

Lab aprons/coats, gloves, and goggles.

Front

What is transformation in the context of microbiology?

Back

The process of introducing foreign DNA into a bacterial cell.

Front

Why is it important to store inoculated plates agar side up?

Back

To prevent contamination and ensure proper growth of bacteria.

Front

What is the role of the pGFP plasmid in the lab?

Back

To allow the E. coli bacteria to express green fluorescent protein.

Front

What precautions should be taken when handling antibiotics in the lab?

Back

Avoid direct participation if allergic and follow safety protocols.

Front

What is the significance of using gloves and goggles in the lab?

Back

To protect against chemical exposure and biological hazards.