MI 2.1.3 Review

Front

What is the purpose of the 100 bp ladder in DNA experiments?

Back

To provide a size reference for DNA fragments.

Front

Which restriction enzyme cannot digest the non-taster allele due to a single base change?

Back

HaeIII.

Front

What is the role of centrifuging in DNA isolation from cheek cells?

Back

To separate the cell debris from the DNA.

Front

What is a point mutation?

Back

A change where one nucleotide is replaced by another, which can result in a Single Nucleotide Polymorphism (SNP).

Front

How many amino acid differences are there between PTC taster and non-taster alleles?

Back

3.

Front

What is a 100 bp ladder used for in gel electrophoresis?

Back

It serves as a molecular weight marker to estimate the size of DNA fragments.

Front

Define Single Nucleotide Polymorphism (SNP).

Back

A variation in a single nucleotide that occurs at a specific position in the genome.

Front

What is the function of restriction enzymes in molecular biology?

Back

To cut DNA at specific sequences, allowing for DNA manipulation and analysis.

Front

Explain the significance of the TAS2R38 gene in taste perception.

Back

It is associated with the ability to taste certain bitter compounds, influencing dietary preferences.

Front

What is the consequence of a mutation in a gene?

Back

It can alter the protein produced, potentially affecting its function.