GEL ELECTROPHORESIS

Front

What is gel electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

Front

What is the primary purpose of gel electrophoresis?

Back

To analyze and compare DNA fragments from different sources.

Front

What is the role of the gel in gel electrophoresis?

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The gel acts as a medium through which the DNA fragments can move; smaller fragments move faster than larger ones.

Front

What type of samples can be analyzed using gel electrophoresis?

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DNA, RNA, and proteins.

Front

What is the significance of the size of DNA fragments in gel electrophoresis?

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The size of DNA fragments determines how far they travel through the gel; smaller fragments travel further than larger ones.

Front

What is the charge of DNA, and how does it affect its movement in gel electrophoresis?

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DNA has a negative charge due to its phosphate backbone, causing it to move towards the positive electrode during electrophoresis.

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What is a DNA ladder in gel electrophoresis?

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A DNA ladder is a mixture of DNA fragments of known sizes used as a reference to determine the size of unknown DNA samples.

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What is the process of staining in gel electrophoresis?

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Staining involves adding a dye to the gel to visualize the DNA fragments after electrophoresis.

Front

What is the difference between agarose gel and polyacrylamide gel?

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Agarose gel is used for separating larger DNA fragments, while polyacrylamide gel is used for smaller fragments and proteins.

Front

What is the role of the buffer solution in gel electrophoresis?

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The buffer solution maintains the pH and provides ions that conduct electricity during the electrophoresis process.