F24 H Molecular Genetics FINAL

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What is the role of the pGLO plasmid in bacterial transformation?

Back

The pGLO plasmid carries the gene for green fluorescent protein (GFP) and the gene for antibiotic resistance, allowing transformed bacteria to grow in the presence of ampicillin and fluoresce under UV light.

Front

What is bacterial transformation?

Back

Bacterial transformation is the process by which bacteria take up foreign DNA from their environment, leading to genetic changes.

Front

What is the expected result in the '+pGLO, LB/amp' plate after incubation?

Back

Bacterial growth without biofluorescence.

Front

What does LB/amp media contain?

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LB/amp media contains Luria-Bertani broth and ampicillin, which supports the growth of bacteria and selects for those that have taken up the pGLO plasmid.

Front

What is the significance of biofluorescence in transformed bacteria?

Back

Biofluorescence indicates the expression of the GFP gene, which is a marker for successful transformation.

Front

What is the function of the ampicillin resistance gene in the pGLO plasmid?

Back

The ampicillin resistance gene allows bacteria that have taken up the plasmid to survive in the presence of ampicillin.

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What is the purpose of using a control plate in bacterial transformation experiments?

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A control plate helps to determine the baseline growth of bacteria without the plasmid, ensuring that any observed effects are due to the transformation.

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What is the difference between selective media and non-selective media?

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Selective media contains substances that inhibit the growth of certain bacteria while allowing others to grow, whereas non-selective media supports the growth of a wide range of bacteria.

Front

What is the role of heat shock in bacterial transformation?

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Heat shock increases the permeability of the bacterial cell membrane, allowing plasmid DNA to enter the cells more effectively.

Front

What is the purpose of incubating the transformed bacteria on ice after heat shock?

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Incubating on ice helps to stabilize the cell membrane and prevent the immediate loss of the plasmid DNA.