Exploring Genomics and Techniques

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What is the function of DNA polymerase in PCR?

Back

DNA polymerase synthesizes new DNA strands by adding complementary nucleotides during the extension phase of PCR.

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What is the main difference between Southern and Northern blotting?

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Southern blotting detects DNA; Northern blotting detects RNA.

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How does hybridization work in molecular biology?

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Hybridization is the binding of complementary nucleic acid strands through base pairing.

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What is the purpose of multiplex PCR?

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The purpose of multiplex PCR is to amplify multiple DNA targets in a single reaction.

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What does RAPD stand for in DNA analysis?

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Random Amplified Polymorphic DNA.

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What is PCR and its significance in genomics?

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PCR (Polymerase Chain Reaction) is a technique used to amplify specific DNA sequences, making it essential for genetic analysis.

Front

What role do primers play in PCR?

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Primers are short sequences of nucleotides that provide a starting point for DNA synthesis during PCR.

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What is the difference between DNA and RNA?

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DNA is double-stranded and contains deoxyribose sugar, while RNA is single-stranded and contains ribose sugar.

Front

What is the purpose of gel electrophoresis in genomics?

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Gel electrophoresis is used to separate DNA fragments based on size, allowing for analysis and comparison.

Front

What is the significance of the Taq polymerase in PCR?

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Taq polymerase is a heat-stable enzyme that synthesizes DNA at high temperatures during PCR.