MI 1.1.3 DNA Sequencing

Front

What charge does DNA have?

Back

DNA has a negative charge.

Front

Which electrode does DNA migrate towards during gel electrophoresis?

Back

DNA migrates towards the positive electrode.

Front

What is the first step in DNA sequencing?

Back

The first step is PCR with fluorescent dideoxynucleotides.

Front

What is the purpose of dideoxynucleotides in DNA sequencing?

Back

Dideoxynucleotides stop the elongation of the growing DNA strand.

Front

How do larger DNA fragments move through a gel compared to smaller fragments?

Back

Larger DNA fragments move through a gel slower than smaller DNA fragments.

Front

Which base pairs correctly match in DNA?

Back

Adenine pairs with Thymine.

Front

What is gel electrophoresis used for in DNA sequencing?

Back

Gel electrophoresis is used to separate DNA fragments by size.

Front

What does PCR stand for?

Back

PCR stands for Polymerase Chain Reaction.

Front

What is the role of lasers and sensors in DNA sequencing?

Back

Lasers and sensors detect fluorescence during DNA sequencing.

Front

What does the BLAST database do in DNA sequencing?

Back

The BLAST database is used to match DNA sequences.