PhysioX Lab 6 Chemical and Physical Processes of Digestion

PhysioX Lab 6-Chemical and Physical Processes of Digestion

Pre Lab Questions

Multiple Choice

Which of the following is true of enzymes?

• They become part of the product

• They are used up in the reaction

• Their activity can be affected by temperature and PH

• They slow down the rate of reaction

Multiple Choice

The reagent IKI tests for the presence of

• starch

• maltose

• glucose

• protein

Multiple Choice

Which of the following is not true of controls?

• With a negative control, a negative result is expected

• With a positive control, a positive result is expected

• A positive result with a negative control could mean that a contamination substance is present

• A negative result with a positive control is required to validate the test

Multiple Choice

Which of the following is an end product of starch digestion?

• amylase

• maltose

• glucose

• amylase and maltose

• maltose and glucose

Multiple Choice

Hydrolases are enzymes that break down large molecules into smaller subunits though the addition of _____

• acid

• water

• salt

• base

Multiple Choice

The substrate for amylase is

• maltose

• starch

• carbohydrate

• maltose and starch

• starch and carbohydrate

Look to the recorded video for the experiment

Post Lab Quiz

look to the recorded video for the

answers of the experimented results

Multiple Choice

Tubes:

3 (amylase, pH 7.0 buffer)

7 (amylase, pH 2.0 buffer)

9 (amylase, pH 9.0 buffer)

reveal that...

• amylase activity was unaffected by pH

• amylase activity was highest at pH 9

• amylase activity was highest at pH 2

• amylase activity was highest at pH 7

Multiple Choice

This activity includes a number of negative controls. Which tube indicates that the amylase solution was not contaminated with maltose?

• tube 1 (amylase, starch, pH 7.0 buffer)

• tube 4 (amylase, deionized water, pH 7.0 buffer)

• tube 6 (deionized water, maltose, pH 7.0 buffer)

• tube 7 (amylase, starch, pH 2.0 buffer)

Multiple Choice

Which tubes indicate that the deionized water did not have contaminating starch or maltose?

• tubes:

  4 (amylase, deionized water, pH 7.0 buffer)

  5 (deionized water, starch, pH 7.0 buffer)

• tubes:

  4 (amylase, deionized water, pH 7.0 buffer)

  6 (deionized water, maltose, pH 7.0 buffer)

• tubes:

  4 (amylase, deionized water, pH 7.0 buffer)

  5 (deionized water, starch, pH 7.0 buffer) and

  6 (deionized water, maltose, pH 7.0 buffer)

• tubes:

  1 (amylase, starch, pH7.0 buffer)

  4 (amylase, deionized water, pH 7.0 buffer)

  5 (deionized water, starch, pH 7.0 buffer)