PhDMicroscopy

​PhD Microscopy pub quiz

Multiple Select

What happens when the light from the transmitted light source hits an aqueous sample at an angle?

• The speed of light changes

• The direction of light changes towards the normal

• The different colors of light are split

• The frequency of light changes

• The direction of light changes away from the normal

Multiple Choice

Why does the red light look green when you approach at high speed?

• Interference between the red waves at different distances from the traffic light changes the wavelength

• The wavemaxima get compressed while you are driving against the speed of light

• Chromatic aberration caused by the windshield diffracts the red light from your eyes

Multiple Choice

What is wrong with this CSI microscope

• Something with the light source

• Something with the oculars

• Something with the objectives

Multiple Select

If there is dirt in your image but not on the sample, where could it be?

• Lamp filament

• Field Diaphragm (Veld diafragma)

• Condensor Diaphragm

• Back Focal Plane (Achterste focale vlak)

• Ocular Diaphragm

Multiple Choice

Who came up with this formula

• Ernst Abbe

• August Kohler

• Albert Einstein

• Victor Schott

• Anthonie van Leeuwenhoek

Multiple Choice

Explain why the amount of fish seems to have doubled?

• You drank too much

• The fish are mirrored at the bottom of the tank

• Refraction causes the light to move in different directions which allows the fish to be portrayed twice

Multiple Choice

Where would you need to aim to inject the fish

• Upper Fish

• Lower fish

• In the middle between the fish

• Below the fish

• Above the fish

Multiple Select

Check all the advantages of selecting a

63x/1.2 N.A. plan-achromat ∞/0.17

objective over a

40x/0.9 160/- N.A. plan-apochromat

• Better resolution

• Brighter image

• Better color correction

• It is easier to use in a fluorescence microscope

• You don't need immersion

Multiple Choice

Which technique produces dark background with a sample that seems to emit light?

• Polarization

• Dark field

• Phase contrast

• DIC

Multiple Choice

Why are you still able to see anything with dark field microscopy if the light falls outside the objective?

• Not all colors are bend equally and therefore some information can be seen

• The reflections of the sample are seen

• We see the 1st order of light being diffracted into the objective

• There is so much light outside you will still see a little bit

Multiple Choice

How do the light waves get separated with DIC

• Using the analyzer

• Using the polarizer

• Using the Wollaston Prism

Multiple Choice

What microscope part is this?

• Scanning mirror

• Dicrhoic mirror

• Porro prism

• Wollaston prism

Multiple Choice

Light is travelling from water to air. Which path will the light most likely follow?

• A

• B

• C

• D

Multiple Choice

Why are stars traditionally represented with 4, 6 or 8 points while they are actually round objects?

• Based on the sun rays, since stars are smaller less rays are depicted

• Due to the diffraction around the diapraghm of the camera

• Our eyes tend to diffract the light more along the horzontal and vertical axis

Multiple Choice

Explain why the sky isn't violet

• Raleigh scattering is less efficient for UV

• Our eyes are less sensitive to UV light

• UV light does not penetrate the earths atmosphere

• Sun light contains more blue light than UV light

Multiple Choice

What is wrong with this microscope?

• It is not kohlered

• Something with the objectives

• Something with the light source

• Something with the oculars

Multiple Select

Why do we need emission filters in a confocal fluorescence microscope?

• The excitation light is too bright otherwise

• To make sure we collect the right color in our detector

• The emission light is too bright otherwise

• To prevent over exposure of the photomultiplier

Multiple Select

How is the emission light of a fluorophore changed in comparison with the excitation light

• It has less energy

• It has a shorter wavelength

• It has a longer wavelength

• The speed has decreased

Multiple Choice

What do we see?

• A spiral of squares

• 5 circles of squares

• Two intertwining spirals

• 4 circles of squares

Multiple Choice

How many megabytes is the size of a 1 Megapixel 8-bit image

• 0.5 Mb

• 1 Mb

• 5 Mb

• 10 Mb

Multiple Select

Name 2 reasons to make 16 bit instead of 8 bit images

• the 8 bit image is over exposed

• The signal is weak and with 16 bit data it can be quantified better

• 16 bit imaging is faster than 8 bit

• 16 bit images are smaller than 8 bit, thereby saving storage space

• The contrast in 16 bit images can be optimized over a larger range of values

Multiple Select

This microscope is used to make pictures of viruses.

What is wrong?

• There is no camera attached to the microscope

• The microscope is not kohlered

• Viruses are too small to be seen with a light microscope

Multiple Select

This is the supposed image from the microscope, what is wrong with the image?

• The scale bar is wrong

• The image is an electron microscopy image instead of a light microscopic image

• For electron microscopy the sample has to be in vacuum