Biotechnology

Multiple Select

Which restriction enzymes from above make blunt cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

Multiple Select

Which restriction enzymes from above make sticky cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

Multiple Choice

What is the purpose of restriction enzymes?

• To join fragments of DNA

• To cut DNA at specific recognition sites

• To amplify DNA sequences

• To separate DNA fragments according to size

Multiple Choice

Why do bacteria have restriction enzymes?

• To prevent being infected by viruses
• To help DNA get into the cell
• To be able to grow in an incubator
• They don't - viruses have restriction enzymes

Multiple Choice

What is the function of DNA ligase?

• To join fragments of DNA

• To cut DNA at specific recognition sites

• To amplify DNA sequences

• To separate DNA fragments according to size

Multiple Choice

What is the function of a plasmid in DNA cloning?

• Amplifying DNA segments

• Cutting DNA at specific sites

• Ligating DNA fragments

• Serving as a vector to carry DNA into host cells

Multiple Choice

What is the term for the resulting DNA molecule when two different DNA fragments are joined together in DNA cloning?

• Recombinant DNA

• Replicated DNA

• Amplified DNA

• Mutated DNA

Multiple Choice

What is the purpose of bacterial transformation in DNA cloning?

• To cut and paste DNA fragments

• To amplify DNA sequences

• To join fragments of DNA

• To introduce foreign DNA into bacteria

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Multiple Choice

What is the goal of PCR?

• To cut DNA at specific recognition sites

• To join fragments of DNA

• To amplify a specific DNA region

• To separate DNA fragments according to size

Multiple Choice

What is denaturation in the PCR process?

• The cooling of the reaction mixture

• The separation of DNA strands by heating

• The addition of primers to the reaction

• The final step in PCR amplification

Multiple Choice

What is the typical temperature range for the denaturation step in PCR?

• 37-42°C

• 50-60°C

• 72-75°C

• 90-95°C

Multiple Choice

What is the purpose of the extension step in PCR?

• To separate DNA strands

• To cool the reaction mixture

• To synthesize new DNA strands

• To add primers to the reaction

Multiple Choice

How many cycles of denaturation, annealing, and extension are typically performed in a PCR reaction?

• 1 cycle

• 5 cycles

• 20-40 cycles

• 90-100 cycles

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Multiple Choice

What is the purpose of gel electrophoresis?

• To cut DNA at specific recognition sites

• To join fragments of DNA

• To amplify DNA sequences

• To separate DNA fragments according to size

Multiple Choice

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Multiple Choice

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged and attracted to the positive end of the unit

• DNA is positively charged and attracted to the negative end of the unit

• the agarose gel is negatively charged

• the agarose gel is positively charged

Multiple Choice

Based on these results whose blood was found in the blood stain at the crime scene?

• Bob
• Sue
• John
• Lisa

Multiple Choice

Which of the following is NOT a component of a DNA sequencing reaction?

• DNA template

• DNA polymerase

• Dideoxynucleotides (ddNTPs)

• Deoxynucleotides (dNTPs)

• Radioactive isotopes

Multiple Choice

What is the role of dideoxynucleotides (ddNTPs) in DNA sequencing?

• They initiate DNA synthesis

• They terminate DNA synthesis at specific bases

• They elongate the DNA strands

• They repair DNA damage