Genetic Engineering

Multiple Choice

Which of the following is NOT a common application of PCR (Polymerase Chain Reaction)?

• DNA analysis for identifying genetic variations

• Medical diagnostics for detecting infections

• Producing proteins like insulin and vaccines

• Forensic science for analyzing crime scene samples

Multiple Select

Which of the following are required components for the amplification of a specific DNA sequence in PCR?

• DNA primers

• dNTPs

• DNA polymerase

• Restriction enzymes

Open Ended

Explain how the thermal cycler is used in the PCR process and why multiple cycles are necessary.

Open Ended

Describe the role of restriction enzymes and DNA ligase in the creation of recombinant DNA.

Multiple Choice

Which of the following statements about plasmids is correct?

• Plasmids are small circular DNA molecules found in bacteria.

• Plasmids cannot replicate on their own.

• Plasmids are used to produce insulin for people with Type 2 Diabetes.

• Plasmids are only found in plants.

Multiple Choice

Which of the following is NOT a reason to compare DNA using gel electrophoresis?

• To determine paternity

• To identify who committed a crime

• To find out how closely species are related

• To increase the length of DNA

Open Ended

Explain how CRISPR technology can be both beneficial and potentially misused in genetic research.

Open Ended

Reflecting on the tools and techniques discussed, which one do you find most interesting or would like to learn more about, and why?