M5_ Molbio

Multiple Choice

Which of the following is a primary role of genetic engineering as discussed in the context of recombinant DNA technology?

• To manipulate DNA experimentally for new traits

• To observe natural DNA without modification

• To prevent any changes in genetic material

• To only study ancient DNA samples

Open Ended

Why is recombinant DNA technology considered significant in the field of molecular biology?

Multiple Choice

Which of the following is NOT a use of recombinant DNA technology?

• Science

• Medicine

• Agriculture

• Transportation

Open Ended

Explain how recombinant DNA (rDNA) differs from naturally occurring DNA sequences.

Multiple Select

Which steps are involved in the process of making recombinant DNA?

• Isolation of DNA

• Cutting DNA with restriction enzymes

• Insertion of foreign DNA into a vector

• DNA replication in the host organism

Fill in the Blanks

The first recombinant DNA was created in

by Herbert Boyer and Stanley Cohen.

Open Ended

Arrange the following steps of rDNA technology in the correct order: Isolation of Genetic Material, Amplification Using PCR, Restriction Enzyme Digestion, Ligation of DNA Molecules, Insertion Into Host, Isolation of Recombinant Cells.

Multiple Select

Which enzymes are used in the isolation of genetic material during rDNA technology?

• Lysozyme

• Cellulase

• Chitinase

• Polymerase

Multiple Choice

Which step in the recombinant DNA process involves the use of molecular scissors to cut DNA at specific sequences?

• Restriction enzyme digestion

• Gel electrophoresis

• Amplification using PCR

• Ligation of DNA molecules

Open Ended

Explain how agarose gel electrophoresis helps in identifying DNA fragments after restriction enzyme digestion.

Multiple Select

Which of the following are essential components required for PCR amplification?

• Template DNA

• DNA ligase

• Primers

• DNA polymerase

Fill in the Blanks

DNA ligase joins DNA fragments to form

DNA molecule.

Open Ended

Describe the process by which recombinant DNA is introduced into a host cell and how host cells are made competent.

Multiple Choice

What is the role of marker genes in the isolation of recombinant cells, and how does the pBR322 plasmid facilitate this process?

• Marker genes help identify successful recombinants

• Marker genes increase transformation efficiency

• Marker genes prevent contamination

• Marker genes replicate independently

Multiple Choice

Which of the following is NOT an application of recombinant DNA technology?

• Production of therapeutic proteins

• Development of genetically modified crops

• Vaccine production

• Antibiotic resistance in bacteria

Multiple Select

Which statements about restriction enzymes are correct?

• They are naturally occurring enzymes from bacteria and viruses.

• They protect bacteria by cutting foreign DNA.

• They were discovered by Stewart Linn & Werner Arber.

• They synthesize new DNA sequences.

Multiple Choice

What is the significance of palindromic sequences in the function of restriction enzymes?

• They allow enzymes to cut DNA at random sites.

• They ensure enzymes cut at specific recognition sites.

• They prevent enzymes from binding to DNA.

• They help enzymes synthesize new DNA.

Fill in the Blanks

A palindromic sequence in DNA is recognized by many restriction enzymes. Fill in the blank: The sequence recognized by EcoRI is

Open Ended

Explain why sticky ends facilitate easier annealing of DNA fragments compared to blunt ends.

Multiple Choice

Which of the following best describes the difference between sticky ends and blunt ends produced by restriction enzymes?

• Sticky ends have single-stranded overhangs, while blunt ends are straight cuts with no overhangs.

• Sticky ends are straight cuts, while blunt ends have overhangs.

• Sticky ends can ligate with any fragment, while blunt ends cannot ligate at all.

• Sticky ends are produced by all restriction enzymes, while blunt ends are not.

Fill in the Blanks

Paul Berg created the first recombinant DNA by combining SV40 monkey virus with

lambda phage.

Fill in the Blanks

Type II restriction enzymes are most commonly used in laboratories because they cut DNA at the

site.

Multiple Select

Which of the following are types of restriction enzymes?

• Type I

• Type II

• Type III

• Type IV

Open Ended

What are two factors that can affect restriction enzyme activity?

Open Ended

Reflecting on today's lesson about recombinant DNA technology, what aspect would you like to explore further or what questions do you still have?

Multiple Choice

Which of the following best describes the importance of genetic engineering as discussed in the lesson?

• It allows for the creation of new species.

• It helps in the detection of recombinant DNA molecules and PCR primers.

• It is only used for experimental manipulation of DNA.

• It has no significant role in molecular biology.