ELISA

What are practical uses for the ELISA test?

• pathogens
• Ebola
• AIDS
• All of the above

What does the S in ELISA stand for?

• substrate
• stationary
• sorbent
• starch

What is a primary antibody?

• Patient's antibody and initial step in ELISA

• Antibody with bound enzyme

• The most important antibody

• a chicken antibody

Give an example of a disease that attacks the immune system.

• Lupus

• Pneumonia

• Strep

• Diabetes

Tags

NGSS.HS-LS1-3

Why is there an enzyme attached to the secondary antibody?

• They link to the proteins used

• The catalyze reactions

• They provide a colormetirc indication of antigen exposure

• They are used to detect DNA

Why did you need to wash the wells after every step?

• To remove unbound antibodies so there will be no false results

• To clean out the wells to see the color change

• Because it is fun

• To target bacteria that may stick to the plastic

Why did you assay your samples in duplicates?

• To act as a control of the assay
• Reproducibility of results
• To test for pipetting errors
• All of the above

When a positive + result occurs in an ELISA, then one could conclude that...

• a + good indication of the patient's health

• the antibody for the particular pathogen is present

• it is a false + and no illness

• an enzyme is present

Antibodies not only protect us from infections, they can also be used in laboratories to determine whether or not a patient has or has had a disease.

• TRUE
• FALSE

Why is a positive and negative control necessary in the setup of ELISA?

• positive controls are equal to negative controls 
• ELISA is subject to errors; if controls fail the results are untrustworthy
• ELISA is a well run test that typically does not need controls
• negative and positive controls are needed to exclude all results