Chapter 9 Biotechnology

A restriction enzyme cuts a segment of DNA into 8 pieces.  How many restriction sites are there?

• 6
• 7
• 8
• 9

A type of restriction map, called a DNA fingerprint, compares the ____ of the ____ of DNA.

• base pairs; noncoding regions
• sizes; coding regions
• lengths; coding regions
• sizes, noncoding regions

Why are primers necessary for PCR?

• They help separate the double helix
• They are a starting point for DNA polymerase
• They help anneal the newly formed strands
• They bring in new nucleotides

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

What is necessary for the polymerase chain reaction to occur?

• DNA polymerase + primers
• RNA polymerase + DNA sequence
• DNA polymerase + mRNA sequence
• RNA polymerase + free floating nucleotides

We can figure out which genes are expressed at the same time by observing

• a DNA fingerprint
• a restriction map
• a microarray
• a piece of recombinant DNA

Genetic engineering involves ___ while gene therapy involves ___.

• a bacterial plasmid, restriction enzymes
• a bacterial plasmid; a virus
• restriction enzymes; a virus
• a virus; a bacterial plasmid

Tags

NGSS.HS-LS3-2

During gel electrophoresis, DNA moves from the ___ end to the ___ end.

• positive, negative
• negative, positive

Compared to sticky ends, which are exposed bases, blunt ends___

• are made of repeating bases
• are paired bases
• are restricted bases
• are made of non complementary bases

To genetically engineer a transgenic organism, the foreign DNA & organism's DNA must contain

• repeating nucleotides
• different nitrogen bases
• the same nitrogenous bases
• noncoding regions of DNA