Microbiology Lab Safety

What is the primary purpose of keeping doors and windows closed during the laboratory session?

• To reduce the incidence of contamination from microbes traveling in the air.
• To keep you all in the room.
• To increase the incidence of contamination from microbes in the room.

After using an inoculating loop or needle to transfer a culture from one medium to the next, what stop should be taken next to avoid contamination.

• Heat the inoculating loop or needle with the bunsen burner flame until it becomes cool to the touch.
• Heat the inoculating loop or needle with the bunsen burner flame until it becomes purple hot.
• Heat the inoculating loop or needle with the bunsen burner flame until it becomes red hot.

When should lab bench tops be cleaned with disinfectant solution?

• Never, take a chance!
• At the beginning and end of lab.
• Only at the end.

It is especially important to use rapid and efficient manipulation to prevent reproductive spore dissemination when handling which type of culture?

• bacterial
• fungal
• viral

Which of the following regulations should be observed in order to avoid injury and infection?

• Never wash hands and keep hair long.
• Wash hands with detergent and ethyl alcohol, tie back long hair, and were closed toed shoes while in the laboratory.
• Wear open toe shoes and drop acid on your feet just for fun!

If you happen to spill a bacterial culture on the floor during lab, which of the following statements describes the correct clean-up procedure?

• Immediately cover the spill with paper towels, saturate with disinfectant, then let sit for 20 minutes.
• Immediately cover the spill with paper towels, saturate with water, then let sit for 20 minutes.
• Immediately cover the spill with paper towels, saturate with disinfectant, then walk away.

It is very important that microbiology students must develop aseptic techniques in order to prepare pure cultures. which of the following statements properly defines aseptic technique?

• A technique that is full of contaminating organisms
• A way to make you get sick when you want to!
• A technique that is free from contaminating organisms

Which of the following laboratory safety statements is incorrect.

• Never eating or drinking in the lab.
• Keep hair pulled back from flames.
• Eating and drinking is allowed in the laboratory as long as open cultures are not on the bench top.

Which of the following statements describes the purpose behind the general rules and regulations of the lab?

• To ensure that you get hurt or sick.
• To prevent accidental injury and infection of yourself and others.
• To ensure that your lab partners are hurt.

Which of the following is an example of proper laboratory technique?

• Do not wear disposable gloves when working with blood, serum, and other body fluids.
• Wear disposable gloves when working with blood, serum, and other body fluids.