Biotechnology

What is the function of naturally occurring restriction enzymes in bacterial cells?

• They are used during DNA replication in the bacterial cell.

• They are used to degrade the bacterial cell's DNA.

• Restriction enzymes recognize and cleave DNA molecules that are foreign to the bacterial cell.

• These enzymes are used to attach pieces of DNA into an opening created by ligase enzymes.

Steps to cloning DNA

Place the following steps in cloning DNA in the correct order

1 - use vector to deliver new rDNA to bacterial or other cells

2 - use restriction enzymes to cut a plasmid and add the desired gene

3 - isolate and cut out a desired gene using restriction enzymes

4 - use DNA ligase to seal the new gene

5 - allow bacterial cells to replicate and produce desired product

• 1, 2, 3, 4, 5

• 5, 3, 2, 4, 1

• 2, 5, 3, 1, 4

• 3, 2, 4, 1, 5

The function of a vector in genetic engineering is to

• cut DNA into many fragments.

• introduce rDNA into a host cell.

• link together newly joined fragments of DNA.

• make millions of copies of a specific segment of DNA.

The term "ligase" adds the suffix "-ase" to the Latin root word for

• gene

• work

• bind

• cut

Which of the following molecules forms lengths of DNA with "sticky ends"?

• restriction enzyme

• DNA polymerase

• RNA polymerase

• DNA ligase

The function of DNA ligase in recombinant technology is to

• cut DNA into many fragments.

• seal DNA into an opening created by restriction enzymes.

• carry DNA into a new cell.

• separate fragments of DNA by their length and electrical charges.

The function of the polymerase chain reaction in genetic engineering is to

• cut DNA into many fragments.

• carry DNA into a new cell.

• link together newly joined fragments of DNA.

• make multiple copies of a specific segment of DNA.

All of the following statements are true about restriction enzymes EXCEPT

• they are made by bacteria and viruses.

• hundreds of different ones have been isolated and purified.

• they produce single-stranded complementary ends that can join to two different DNA strands by complementary base pairing.

• each enzyme cuts DNA at a different specific base sequence.

What was the minimal requirement to perform a "classic DNA fingerprinting" test in order to associate a blood sample with a criminal?

• one sample of DNA, PCR amplification, restriction enzymes, and gel electrophoresis

• one sample of DNA, restriction enzymes, a DNA synthesizer, and gel electrophoresis

• two samples of DNA, PCR amplification, restriction enzymes, and gel electrophoresis

• two samples of DNA, PCR amplification, a DNA synthesizer, and gel electrophoresis

To carry out a polymerase chain reaction (PCR), you must have DNA polymerase and

• a blueprint or gene map of the sequence you wish to copy.

• the nucleotides to synthesize new DNA strands.

• a DNA synthesizer machine.

• a DNA probe.