Insertional Inactivation

Insertional inactivation is defined as

• technique to identify desirable gene

• technique to recognise non-recombinant cells

• technique to recognise recombinant cells

• technique to recognise specific cell types

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

• insertional inactivation of β-galactosidase in recombinant bacteria

• inactivation of glycosidase in recombinant bacteria

• recombinant bacteria containing β-galactosidase

• insertional inactivation of α-galactosidase in non-recombinant bacteria

The alternative selectable marker and chromogenic substrate that selects recombinants on the basis of their ability to produce colour are

• Lac-Z gene and X-gal

• α-galactosidase and X-gal

• Amp R gene and X-gal

• β-galactosidase and glucose

An antibiotic resistance gene in a vector helps in the selection of

• competent bacterial cells

• transformed bacterial cells

• recombinant bacterial cells

• none of the above

In genetic engineering, the antibiotics are used

• as sequences from where replication starts

• to keep the cultures free of infection

• as selectable markers

• to select healthy vectors

Which vector can clone only a small fragment of DNA

• Bacterial artificial chromosome

• Yeast artificial chromosome

• Plasmid

• Cosmid

The term molecular scissors generally refers to

• DNA polymerases

• RNA polymerases

• Restriction endonucleases

• DNA ligases

The restriction enzyme ECOR1 has the property of

• endonuclease activity

• exonuclease activity

• ligation activity

• correcting the topology of replicating DNA

In r-DNA technique which of the following technique is not used in introducing DNA into host cell

• transduction

• conjugation

• transformation

• electroporation

What is true for plasmid

• Plasmids are widely used in gene transfer

• These are found in viruses

• Plasmid contains gene for vital activities

• These are main part of chromosome