CHROMATOGRAPHY

which of the following is correct statement

• Resolution is proportional to the square root of the number of theoretical plates in a column

• Resolution is inverse proportional to the square root of the number of theoretical plates in a column

• Resolution is proportional to the square of the number of theoretical plates in a column

• Resolution is proportional to the number of theoretical plates in a column E. Resolution is inverse proportional to the number of theoretical plates in a column

In GC, retention depends upon the

• Vapor pressure and polarity of the solute

• Size and charge of the solute

• The method of sample introduction

• The type of detector used

• Type of column used

Peak broadening is greatly contributed by

• Column fitting

• Mobile phase flow rate/ viscosity

• Stagnant mobile phase in column

• Detector sensitiveness

• Stationary phase nature

In reverse phase chromatography, the stationary phase is made

• non-polar

• polar

• either non-polar or polar

• none of these

. Ion exchange chromatography is based on the

• electrostatic attraction

• electrical mobility of ionic species

• adsorption chromatography

• partition chromatography

In gas chromatography, the basis for separation of the components of the volatile material is the difference in

• partition coefficients

• conductivity

• molecular weight

• molarity

Thin layer chromatography is

• partition chromatography

• electrical mobility of ionic species

• adsorption chromatography

• none of the above

A characteristic feature of any form of chromatography is the ...

• calculation of an Rf value for the molecules separated.

• use of an inert carrier gas.

• use of a mobile and a stationary phase.

• use of molecules that are soluble in water.

Which of the following statements about chromatography is correct?

• Paper chromatography is usually considered to be quantitative only, while gas chromatography can be qualitative or quantitative.

• Paper chromatography and gas chromatography are both routinely used for qualitative analysis only.

• Paper chromatography and gas chromatography are both routinely used for quantitative analysis only.

• Paper chromatography is usually considered to be qualitative only, while gas chromatography can be qualitative or quantitative.

In gas chromatography, the concentration of a substance can be determined by ...

• comparison of the Rt of the substance with that of a standard.

• measurement of the height of the peak produced by the substance.

• comparison of the area under the peak produced by the substance with the areas under the peaks produced by standard solutions.

• calculation of the Rt value of the substance.