3.5 IB Restriction Endonucleases

If the target sequence or restriction site is ^5'GCTTCG^3', what is the complementary sequence read in the 3'-5' direction?

• CGAAGC

• GCTTCG

• CGUUCG

• GCUUCG

What is a restriction enzyme?

• An enzyme that cuts DNA

• An enzyme that add to the DNA strand

• An enzyme that builds proteins

• An enzyme that breaks down lipids

A special protein that targets a specific base sequence and cuts DNA into smaller fragments.

• PCR

• DNA Polymerase

• Helicase

• Restriction Enzyme

What is the term given to symmetrical ends of DNA after being cut by a restriction enzyme?

• Blunt Ends

• Sticky Ends

• Ligated Ends

• Restrictive Ends

This biomolecule originates from bacterial DNA and has the ability to cut DNA....

• Helicase

• Ligase

• Restriction Enzyme

• DNA Polymerase

DNA cutting "scissors" are more correctly referred to as....

• Bacteriophages

• Restriction Enzymes

• DNA Enzymes

• Plasmids

The unpaired nucleotides produced by the action of restriction enzymes are referred to as.....

• sticky end.

• base sequence.

• single strands.

• restriction fragments.

The recognition sites for restriction enzymes are usually...

• alliterations

• pallindromes

• acronyms

• analogies

Restriction Enzymes are isolated from....

• proteins

• bacteria

• viruses

• bacteriophages

Restriction Enzymes were primarily used as....

• defense by protozoa against antibiotics

• tools by viruses to cleave bacterial genome

• defense by bacteria against bacteriophages viruses

• tools to cleave DNA during replication