lecture 29

select all ways to elucidate metabolic pathways?

• interrupt pathway and look for changes in intermediates

• use chemical inhibitors to block the pathways

• use mutations that inactivate the specific enzymes

• use a lot of reactants

• Observe the transient passage of molecules through pathways

Heavy Atom isotopes

• can be used to label nutrients and then observed

• can be observed with NMR

• not useful in elucidating metabolic pathways

define beta isotope

• electron emitted from nucleus

• positron (antielectron)

• high energy photon

define beta positive isotope

• electron emitted from nucleus

• positron (antielectron)

• high energy photon

define gamma isotope

• electron emitted from nucleus

• positron (antielectron)

• high energy photon

¹⁴C is what type of radioactive isotope?

• beta

• beta positive

• gamma

• beta, gamma

• beta positive, gamma

³H is what type of radioactive isotope?

• beta

• beta positive

• gamma

• beta, gamma

• beta positive, gamma

²²Na is what type of radioactive isotope?

• beta

• beta positive

• gamma

• beta, gamma

• beta positive, gamma

⁶⁰Co is what type of radioactive isotope?

• beta

• beta positive

• gamma

• beta, gamma

• beta positive, gamma

1D NMR spectrum can quantify relative amounts of different metabolites

• true

• false