Agarose & Polyacrylamide Gel Electrophoresis

The Diagram below represents the results of a procedure that seperated the DNA in each sample. Results of gel electrophoresis of four organisms, bottom band labelled as band X. What does band X represents?

• Largest fragment of DNA that travelled the slowest

• Smallest fragment of DNA that travelled the slowest

• Smallest fragment of DNA that travelled the fastest

• Largest fragment of DNA that travelled the fastest

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

• Convert the DNA into gel

• Cut the DNA into fragments

• Change the colour of DNA

• Produce longer sections of DNA

What applications can agarose gel electrophoresis be used for?

• Parental Testing

• Sequence Endangered Species DNA

• Criminal Investigation

• All of the above

What is Ethidium Bromide?

• Buffer

• Dye

• DNA solution

• Restriction enzyme

Gel electrophoresis is used to separate DNA fragments on the basis of which property?

• size

• color

• functions

• chromosomes

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

During gel electrophoresis, DNA moves from the ___ end to the ___ end.

• positive, negative
• negative, positive

Which type of gel electrophoresis has higher resolving power?

• Agarose gel electrophoresis

• Polyacrylamide gel electrophoresis

In an SDS-PAGE experiment, proteins are seperated on the basis of their

• Negatively charged side chain

• Different Isoelectric Point

• Molecular Weight

• Change to mass ratio

In an SDS-PAGE gel,

• Proteins are denatured by SDS

• Proteins have same charge to mass ratio

• Smaller proteins migrate more rapidly through the gel

• All of the above