RECOMBINANT DNA TECHNOLOGY

Which of the following is a circular DNA from bacteria that can hold a foreign gene?

• plasmid
• restriction enzyme
• ligase
• protein expression

Which of the following is a protein that acts as DNA scissors?

• plasmid
• restriction enzyme
• ligase
• protein expression

Which of the following is an enzymatic protein that join DNA fragment between gene of interest and plasmid?

• plasmid

• restriction enzyme

• ligase

• protein expression

Which is true about plasmids?

• Plasmids can be cut at specific sequences called restriction sites.
• Plasmids contain a promoter sequence that defines where transcription begins.
• Plasmids can contain an antibiotic resistance gene.
• All of the statements are true about plasmids.

What is recombinant DNA?

• DNA containing genes foreign to that organism

• DNA which has been impacted by epigenetics

• DNA that has restriction enzymes

• DNA that has been sequenced

Restriction enzymes....

• Cut DNA at a specific site

• Can be used to cleave gene of interest

• Can be used to cut vector DNA

• Do all of these things

Restriction enzymes....

• Cut DNA at a specific site

• Can be used to cleave gene of interest

• Can be used to cut vector DNA

• Do all of these things

A struture of plasmid that contain gene of interest are called?

• nonrecombinant plasmid

• recombinant DNA

• binary

• selective species

Tags

NGSS.HS-LS1-1

A ____________ is required to transfer genes from one organism to another.

• vector
• reverse transcriptase
• transport molecule
• genetic probe

Cell that receive recombinant DNA for cloning purpose

• Host cell

• plasmid

• DNA target

• Gene of interest