Recombinant DNA Technology

Recombinant DNA technology

• is possible only between closely related species

• uses bacteria to make copies of the desired product

• uses bacteria to make copies of the desired product and splices DNAs together.

• uses bacteria to make copies of the desired product, splices DNAs together, and is possible only between closely related species

Through natural mechanisms, recombination occurs during crossing over between

• members of the same species.

• members of different species.

• homologous chromosomes

• base pairs on any adjacent chromosomes

The goal of genetic engineering is to?

• eliminate antibiotic resistance in bacteria

• isolate disease-causing plasmids

• transfer of viral genes

• modify cells to correct a defect or produce a desired product

These are the main tools used in recombinant DNA technology except

• host cells

• cosmid

• restriction endonuclease

• DNA polymerase

In order for DNA molecules to undergo recombination they

• they must be from the same species

• their strands must separate as in replication

• must be cut and spliced at specific nucleotide sequences

• undergo lysis

Enzymes used to cut genes in recombinant DNA research are

• ligases

• restriction enzymes

• transcriptases

• DNA polymerases

The “natural” use of restriction enzymes by bacteria is to

• integrate viral DNA

• destroy viral DNA

• repair “sticky ends"

• copy the bacterial genes

Which of the following organisms produce restriction enzymes?

• Bacteria

• Yeast

• Retrovirus

• Bacteriophage

The restriction site recognized by EcoRI is

• GGCATT

• GAATTC

• GCCATT

• GGATCC

The restriction site recognized by BamHI is

• GGCATT

• GCCATT

• GAATTC

• GGATCC