Microbiology Lab Practical
Authored by Bianca Bryant
Biology
University
NGSS covered
Used 115+ times
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This microbiology lab practical focuses on bacterial identification through biochemical testing and represents college-level coursework appropriate for introductory microbiology students. The quiz comprehensively covers two major categories of bacterial testing: enzyme activity tests (amylase, caseinase, DNase, and lipase) and differential/selective media applications (EMB agar, MacConkey's agar, and Mannitol Salt Agar), along with the IMVIC test series (Indole, Methyl Red, Voges-Proskauer, and Citrate utilization). Students must demonstrate mastery of the relationships between specific substrates, their corresponding growth media, enzymatic breakdown products, and the reagents used to detect positive or negative results. The content requires students to understand biochemical pathways, interpret color changes and zone formations, and connect bacterial metabolic capabilities to identification protocols used in clinical and research laboratories. Created by Bianca Bryant, a Biology teacher in US who teaches grade 13. This comprehensive assessment serves as an excellent tool for evaluating student readiness for practical laboratory examinations, making it ideal for pre-lab preparation, post-lab review, or formative assessment during the bacterial identification unit. The quiz structure mirrors the decision-making process students encounter during actual bench work, where they must select appropriate media, predict expected results, and interpret outcomes to identify unknown bacterial specimens. Teachers can deploy this as homework to reinforce theoretical concepts before hands-on lab sessions, use it as a warm-up activity to activate prior knowledge, or implement it as a review tool before summative practical exams. The content aligns with college-level microbiology learning objectives that emphasize practical application of bacterial identification techniques essential for students pursuing careers in healthcare, biotechnology, or advanced biological research.
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38 questions
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1.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of EMB agar?
differentiate between staphylococcus aureus and streptococci
test for casein
test for ds DNA
test for lipid
selective staining for gram-negative bacteria
2.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of Amylase Test?
test for starch
test for casein
test for ds DNA
test for lipid
selective staining for gram-negative bacteria
3.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of lipase test?
differentiate between staphylococcus aureus and streptococci
test for casein
test for ds DNA
test for lipid
selective staining for gram-negative bacteria
4.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of DNase test?
differentiate between staphylococcus aureus and streptococci
test for casein
test for ds DNA
test for lipid
selective staining for gram-negative bacteria
5.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
What is the purpose of Caseinase test?
differentiate between staphylococcus aureus and streptococci
test for casein
test for ds DNA
test for lipid
selective staining for gram-negative bacteria
6.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which of the following medium is used for Amylase testing?
starch agar
skim milk agar
DNase agar
spirit blue agar
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Which of the following medium is used for Caseina testing?
starch agar
skim milk agar
DNase agar
spirit blue agar
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