SDS-PAGE

The matrix used in SDS-PAGE is the same material used for common agarose gel for DNA separation

• True

• False

The gel-position for agarose-gel and SDS-PAGE is horizontal and vertical, respectively.

• True

• False

In agarose gel, bands move to +ve changed electrode. However, in SDS-PAGE, bands move to -ve charged electrode.

• True

• False

Sodium Dodecyl Sulfate is a chemical that can reduce S=S bond

• True

• False

SDS-PAGE can be used to determine protein purity of a given sample

• True

• False

A heterodimer enzyme-X consists of a subunit of 20kD and a subunit of 35 kDa. What is the size of band that will appear on modified SDS-PAGE (without Beta-mercaptoethanol treatment)?

• two bands of 20kD and 35kD, respectively

• One band with 55 kDa

• Three bands with 20kD, 35kD, and 55 kDa, respectively

• Smear because separation will be poor

A complex enzyme-S consists of two subunits of 10kD each, and two subunits of 20 kDa each. What is the size of band that will appear on SDS-PAGE (with Beta-mercaptoethanol treatment)?

• bands with 10kD and 20kD, respectively

• bands with 20kD and 40kD, respectively

• One band with 30 kDa

• One band with 60 kDa

After electrophoresis has completed, the band will appear after straining in Coomassie blue solution.

• True

• False

After breaking a culture of E. coli, the extracted protein is run in a SDS-PAGE. Guess what will you see?

• Single band

• Multiple bands

Coomassie blue has another name. It is....

• Smart Blue

• Brilliant Blue

• Superb Blue