Protein Assay and Enzyme assay

At _____nm wave length, we can roughly measure the concentration of protein.

• 220

• 230

• 260/280

• 280

• 260/230

Quantification of protein using a UV spectrophotometer is based on ____

• UV light is absorbed by the backbone of the protein

• UV light is absorbed by the peptide bonds of the protein

• UV light is absorbed by the aromatic amino acids of the protein

• UV light is absorbed by all the non-aromatic amino acids of the protein

Biuret assay is based on ____ interaction with protein

• Cu2+

• Brillant blue

• Folin-Ciocalteu reagent

The primary component in Biuret assay detects ______ of the proteins

• Amino acids

• Peptide bonds

Bradford Assay is based on ____ interaction with protein

• Cu2+

• Coomassie Blue

• Folin-Ciocalteu reagent

A particular enzyme assay measures____

• The depletion of substrate and the formation of the product at the same time

• The depletion of substrate or the formation of the product

• The formation of intermediate of the reactions

• The concentration of the enzyme

Which of the following selections could be correct? (you can select multiple answers). 1 Unit of X-enzyme is_______

• the amount of enzyme that can form 1 nano-mole of P per min per ml

• the amount of enzyme that can hydrolyse 10% of substrate per min per ml

• the amount of enzyme that can stabilise the substrate per min per ml

Tags

NGSS.HS-PS1-5

The concentration of an 'unknown protein' is usually compare with a standard curve with Y-axis is OD, and X-axis is the protein concentration

• True

• False

The activity of an 'unknown protein' is usually compare with a standard curve with Y-axis is OD, and X-axis is the product concentration

• True

• False