Michaelis Menten Kinetics

This equation is used to describe the kinetics of an enzymatic reaction. The equation is called the

• Inhibitor Equation

• Activation energy equation

• Velocity equation

• Michaelis-Menten equation

• Double-reciprocal plot

What would a chemical equation of two substrates going to produce one product? (select all that apply)

• A + B + E $\leftrightarrow$ ABE $\leftrightarrow$ E + P

• A + B + E $\leftrightarrow$ ABE $\leftrightarrow$ E + P + Q

• 2A + E $\leftrightarrow$ 2AE $\leftrightarrow$ E + P

• All of the equations above are correct

What is "C" on the graph?

• Vmax

• Km

• Vi

• [S]

• Rate of reaction

What is "D" on the graph

• Vmax

• Km

• Vi

• Rate of reaction

• [S]

What is "A" on the graph? (Select all that may apply)

• Km

• Vmax

• Rate of reaction

• [S]

• Vi

What is "B" on the graph?

• Km

• [S]

• Vmax

• Rate of reaction

• Vmin

Vmax = (choose best answer)

• The concentration of substrate that = 1/2 of the Km

• The concentration specified in moles/liter

• The maximum velocity at which all the enzyme's active sites are saturated

• The initial velocity before the enzyme produces a product

What factors would affect the reaction rate?

• Temperature

• pH

• Concentration of inhibitors

• All of the above

Km = (choose best answer)

• The initial velocity prior to enzymatic activity

• The concentration of substrate (moles/liter) needed to achieve 50% of the maximum

• The concentration of substrate at which all enzymes are being used (saturated) and it is producing at maximum effect.

• It represents the first order portion of the graph

Qualitative features of Michaelis Menten Equation is

• The reaction rate is 1st order

• As substrate concentration increases reaction order in substrate decreases

• The rate of reaction is proportional to the total amount of enzyme present

• All the above