Pharmaceutical Biotechnology Sessional

The role of restriction enzymes in DNA technology is to

• provide a vector for the transfer of recombinant DNA

• produce cDNA from mRNA

• produce a cut (usually staggered) at specific restriction sites on DNA

• reseal "sticky ends" after base pairing of complementary bases

Yeast has become important in genetic engineering because it

• has plasmids that can be genetically engineered

• facilitates the study of eukaryotic gene regulation and expression

• grows readily and rapidly in the laboratory

• does all of the above

Gel electrophoresis is a means of separating fragments of DNA based on

• charge

• nucleotide sequence

• genetic information

• size

• both a and d

Two DNA fragments created by cutting both with the same restriction enzyme can be recombined

• if both DNA fragments come from the same species

• if both DNA fragments come from animals

• if both DNA fragments come from eukaryotes

• regardless of the sources of the DNA

Which is the first enzyme used in the production of a recombinant plasmid?

• restriction enzyme

• reverse transcriptase

• DNA ligase

• DNA polymerase

Which is the last enzyme involved in making recombinant plasmids?

• restriction enzymes

• reverse transcriptase

• DNA ligase

• DNA polymerase

Which is the first enzyme used in the production of cDNA?

• restriction enzyme

• reverse transcriptase

• DNA ligase

• DNA polymerase

Which enzyme is used in PCR?

• restriction enzyme

• reverse transcriptase

• DNA ligase

• DNA polymerase

Which of the following has the greatest potential for generating different cell types?

• ES cells

• iPS cells

• adult stem cells

• reprogrammed differentiated body cells

Which of the following would be useful in signaling the presence of a disease-causing allele even if the gene has not yet been identified?

• RNA interference

• in situ hybridization

• single nucleotide polymorphisms

• RT-PCR