What is chromatography?

The method through which solutes are separated

The process by which particles move from a region of higher concentration to lower concentration

The process by which water is purified

In Chromatography the retardation factor R f are used for identification of components. A high R f and a low R t (retention time) mean the component has a

greater affinity to the mobile phase in both TLC and HPLC

greater affinity to the stationary phase in both TLC and HPLC.

greater affinity to the stationary phase in TLC but a greater affinity for the mobile phase in HPLC

greater affinity to the mobile phase in TLC but a greater affininty for the stationary phase in HPLC

Which of the following best describes the principle of chromatography?

Different substances move at different rates

All substances move at the same rate

Only colored substances can be separated

Chromatography is only used in chemistry

How does the stationary phase function in chromatography?

It remains fixed and interacts differently with components

It provides the solvent for the mobile phase

It detects the components after separation

It moves with the mobile phase to separate components

What is the first step in the chromatography process?

Development of the mobile phase

What happens during the development step of chromatography?

The mobile phase moves through the stationary phase

The sample is applied to the stationary phase

The components are detected and analyzed

The stationary phase is prepared for use

What is the main purpose of the detection step in chromatography?

To analyze the individual components after separation

To prepare the mobile phase for movement

To separate the components of the mixture

To apply the sample onto the stationary phase

Which type of chromatography is best suited for separating volatile substances?

High-Performance Liquid Chromatography

How does High-Performance Liquid Chromatography (HPLC) differ from other types of chromatography?

It uses high pressure to push the mobile phase

It only separates colored substances

It is the simplest form of chromatography

It requires a solid stationary phase

What is a common application of chromatography in environmental science?

Detecting pollutants in water and air samples

What is the primary purpose of Thin Layer Chromatography (TLC)?

To separate and analyze mixtures of substances

To measure the temperature of substances

What is the main advantage of using Thin Layer Chromatography (TLC) in laboratories?

It is simple, fast, and low cost

It requires expensive equipment

In the context of TLC, what does the term 'stationary phase' refer to?

The solid layer that holds the compounds

The solvent that moves the sample

In TLC, what is the role of the mobile phase?

To carry the compounds up the plate

To prepare the stationary phase

What happens to components of a mixture during the TLC process?

They separate based on their interaction with the stationary phase

They dissolve completely in the mobile phase

They become invisible during the process

What visualization method might be used if the spots on a TLC plate are not visible?

Using UV light or a staining solution

Measuring the distance traveled

How is the Rf value calculated in TLC?

Distance traveled by the compound divided by distance traveled by the solvent

Distance traveled by the solvent divided by distance traveled by the compound

Total distance traveled by all compounds

Average distance traveled by the sample

If a chemist wants to separate colors in black ink using TLC, what is the first action they should take?

Spread a thin layer of silica gel on a glass plate

Measure the distance traveled by the solvent

Chromatography

Authored by P Lalitha

Science

University - Professional Development

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36 questions

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MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Two or more substances that are mixed together but not chemically combined is a ___

compound

mixture

keratin

chromatography

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

chromatography separates the mixture of dyes on the basis of their ----------------------------

density

solubility

gravity

boiling point

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The substance in the mixture that has the greatest affinity for the stationary phase will travel

fastest

slowest

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The retention factor is used

to zero the chromatography scale

to identify unknown compounds in a sample

to retain solvent

as a starting tool for chromatography

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

The mobile phase in paper chromatography is typically a

plasma

solid

gas

liquid

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which forms of Chromatography use R_tvalues?
I paper chromatoraphy
II TLC
III HPLC
IV GC

I and II

III and IV

II only

III only

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

When using water as a solvent which type of molecule is likely to have the highest R_fvalue

polar

non-polar

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Chromatography

Two or more substances that are mixed together but not chemically combined is a ___

chromatography separates the mixture of dyes on the basis of their ----------------------------

The substance in the mixture that has the greatest affinity for the stationary phase will travel

The retention factor is used

The mobile phase in paper chromatography is typically a

Which forms of Chromatography use R_tvalues?
I paper chromatoraphy
II TLC
III HPLC
IV GC

When using water as a solvent which type of molecule is likely to have the highest R_fvalue

What is chromatography?

Diffusion is non-existent in ___________?

Descending chromatography is favoured by ________________?

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Chromatography

Two or more substances that are mixed together but not chemically combined is a ___

chromatography separates the mixture of dyes on the basis of their ----------------------------

The substance in the mixture that has the greatest affinity for the stationary phase will travel

The retention factor is used

The mobile phase in paper chromatography is typically a

Which forms of Chromatography use Rt values?I paper chromatoraphyII TLCIII HPLCIV GC

When using water as a solvent which type of molecule is likely to have the highest Rf value

What is chromatography?

Diffusion is non-existent in ___________?

Descending chromatography is favoured by ________________?

Access all questions and much more by creating a free account

Similar Resources on Wayground

Popular Resources on Wayground

Discover more resources for Science

Which forms of Chromatography use R_tvalues?
I paper chromatoraphy
II TLC
III HPLC
IV GC

When using water as a solvent which type of molecule is likely to have the highest R_fvalue